Different contributions of nonmuscle myosin IIA and IIB to the organization of stress fiber subtypes in fibroblasts

Masahiro Kuragano, Taro Q.P. Uyeda, Keiju Kamijo, Yota Murakami, Masayuki Takahashi*, Yu Li Wang

*この研究の対応する著者

研究成果: Article査読

14 被引用数 (Scopus)

抄録

Stress fibers (SFs) are contractile, force-generating bundled structures that can be classified into three subtypes, namely ventral SFs (vSFs), transverse arcs (TAs), and dorsal SFs. Nonmuscle myosin II (NMII) is the main component of SFs. This study examined the roles of the NMII isoforms NMIIA and NMIIB in the organization of each SF subtype in immortalized fibroblasts. Knockdown (KD) of NMIIA (a major isoform) resulted in loss of TAs from the lamella and caused the lamella to lose its flattened shape. Exogenous expression of NMIIB rescued this defect in TA formation. However, the TAs that formed on exogenous NMIIB expression in NMIIA-KD cells and the remaining TAs in NMIIB-KD cells, which mainly consisted of NMIIB and NMIIA, respectively, failed to rescue the defect in lamellar flattening. These results indicate that both isoforms are required for the proper function of TAs in lamellar flattening. KD of NMIIB resulted in loss of vSFs from the central region of the cell body, and this defect was not rescued by exogenous expression of NMIIA, indicating that NMIIA cannot replace the function of NMIIB in vSF formation. Moreover, we raised the possibility that actin filaments in vSFs are in a stretched conformation.

本文言語English
ページ(範囲)911-922
ページ数12
ジャーナルMolecular biology of the cell
29
8
DOI
出版ステータスPublished - 2018 4月 15

ASJC Scopus subject areas

  • 分子生物学
  • 細胞生物学

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