Different target-site specificities of the hairpin ribozyme in cis and trans cleavages

Kazuko Fujitani, Noriko Sasaki-Tozawa, Yo Kikuchi

研究成果: Article

5 引用 (Scopus)

抜粋

The hairpin ribozyme cleaves a phosphodiester bond at the 5' side of a 5'GUC3' sequence of an RNA with high efficiency. An RNA having a 5'GUA3' sequence instead of the GUC sequence is a poor substrate for this ribozyme. Here, we show that this is indeed so in a trans-acting ribozyme system, but in a cis-acting ribozyme system this ribozyme cleaves the 5' side of a GUA sequence as efficiently as the wild-type cleaves the GUC sequence. One base substitution in the ribozyme also affected the target-site specificity in the cis-acting system.

元の言語English
ページ(範囲)155-158
ページ数4
ジャーナルFEBS Letters
331
発行部数1-2
DOI
出版物ステータスPublished - 1993 9 27
外部発表Yes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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