Differential neurite outgrowth is required for axon specification by cultured hippocampal neurons

Hideaki Yamamoto, Takanori Demura, Mayu Morita, Gary A. Banker, Takashi Tanii, Shun Nakamura

研究成果: Article査読

47 被引用数 (Scopus)

抄録

Formation of an axon is the first morphological evidence of neuronal polarization, visible as a profound outgrowth of the axon compared with sibling neurites. One unsolved question on the mechanism of axon formation is the role of axon outgrowth in axon specification. This question was difficult to assess, because neurons freely extend their neurites in a conventional culture. Here, we leveraged surface nano/micro-modification techniques to fabricate a template substrate for constraining neurite lengths of cultured neurons. Using the template, we asked (i) Do neurons polarize even if all neurites cannot grow sufficiently long? (ii) Would the neurite be fated to become an axon if only one was allowed to grow long? A pattern with symmetrical short paths (20 μm) was used to address the former question, and an asymmetrical pattern with one path extended to 100 μm for the latter. Axon formation was evaluated by tau-1/MAP2 immunostaining and live-cell imaging of constitutively-active kinesin-1. We found that (1) neurons cannot polarize when extension of all neurites is restricted and that (2) when only a single neurite is permitted to grow long, neurons polarize and the longest neurite becomes the axon. These results provide clear evidence that axon outgrowth is required for its specification. Axon formation: no growth, no specification One unsolved question on the mechanism of axon formation by cultured neurons was the role of axon outgrowth in axon specification. Using micropatterned surfaces to extrinsically regulate lengths of individual neurites, we show that differential outgrowth of a future axon is required for axon specification. The results presented here provide clear evidence that an axon cannot be specified without sufficient outgrowth.

本文言語English
ページ(範囲)904-910
ページ数7
ジャーナルJournal of neurochemistry
123
6
DOI
出版ステータスPublished - 2012 12

ASJC Scopus subject areas

  • 生化学
  • 細胞および分子神経科学

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