Differentiation elicits negative regulation of human β-galactoside α2,6-sialyltransferase at the mRNA level in the HL-60 cell line

Akiyoshi Taniguchi*, Koji Higai, Yuko Hasegawa, Kunio Utsumi, Kojiro Matsumoto

*この研究の対応する著者

研究成果: Article査読

24 被引用数 (Scopus)

抄録

We studied the regulation of the β-galactoside α2,6-sialyltransferase (hST6Gal I) gene during HL-60 cell differentiation induced with dimethyl sulfoxide (DMSO), all trans-retinoic acid (ATRA), and phorbol myristate acetate (PMA). During HL-60 cell line differentiation, cell surface levels of α2,6-sialic acids expression decreased, as measured by flow cytometric analysis using Sambucus nigra agglutinin (SNA). Activities of hST6Gal I and levels of hST6Gal I mRNA dramatically decreased after 1 day of stimulation. Using reverse transcription polymerase chain reaction (PT-PCR), we found the major hST6Gal I mRNA isoform in HL-60 cells contains 5'-untranslated exons Y and Z. These results suggest that the expression of cell surface α2,6-sialic acids is controlled at the mRNA level, which is regulated by a promoter located 5'-upstream of exon Y. Copyright (C) 1998 Federation of European Biochemical Societies.

本文言語English
ページ(範囲)191-194
ページ数4
ジャーナルFEBS Letters
441
2
DOI
出版ステータスPublished - 1998 12月 18
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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