Differentiation of pinopsin-immunoreactive cells in the developing quail pineal organ: An in-vivo and in-vitro immunohistochemical study

Mikaru Yamao, Masasuke Araki*, Toshiyuki Okano, Yoshitaka Fukada, Tadashi Oishi

*この研究の対応する著者

研究成果: Article査読

18 被引用数 (Scopus)

抄録

The avian pineal organ contains several types of photoreceptors with different photopigments: rhodopsin, iodopsin, and pinopsin. We have previously examined the differentiation of both rhodopsin-like and iodopsin-like immunoreactive cells during pineal development in quail embryos to determine the onset of synthesis of specific proteins and their cellular localization. In the present study, we have performed pinopsin immunohistochemistry on in-vivo developing and in-vitro cultured pineal organs of quail embryos. The results were corn pared with those obtained with rhodopsin and iodopsin immunohistochemistry. In the developing pineal organs, pinopsin immunoreactivity was detected at embryonic day 8, i.e. five days earlier than rhodopsin-like and iodopsin-like immunoreactivities. It was localized exclusively in the protrusions extending into the lumen throughout development, whereas rhodopsin-like and iodopsin-like immunoreactivities were usually found both in cell bodies and processes. These differences were also observed under two different types of culture conditions (dissociated cell culture and organ culture) indicating that, in the avian pineal organ, the expression pattern of the pinopsin gene is basically different from those of the other two pineal photopigments. The present study suggests that pineal cells have a mechanism for the polarized transport of pinopsin molecules.

本文言語English
ページ(範囲)667-671
ページ数5
ジャーナルCell and Tissue Research
296
3
DOI
出版ステータスPublished - 1999
外部発表はい

ASJC Scopus subject areas

  • 病理学および法医学
  • 組織学
  • 細胞生物学

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