Direct Count of Bacteria Using Fluorescent Dyes: Application to Assessment of Electrochemical Disinfection

Tadashi Matsunaga, Mina Okochi, Satoshi Nakasono

研究成果: Article査読

37 被引用数 (Scopus)

抄録

A rapid method for direct counting of viable and dead cells using two fluorescent dyes, propidium iodide and 4 ', 6-diamidino-2-phenylindole, was developed for estimation of cell viability after electrochemical disinfection. Using this double staining method, dead and viable cells were visualized as red and blue fluorescent cells, respectively, under UV excitation employing epifluorescence microscopy. A good correlation between the red cell ratio and the ratio of dead cells determined by the plate count method was obtained for Vibrio alginolyticus cells exposed to heat (60 °C), 10 ppm of chlorine, 3.7% of formaldehyde, and electrochemical disinfection treatment.

本文言語English
ページ(範囲)4487-4490
ページ数4
ジャーナルAnalytical Chemistry
67
24
DOI
出版ステータスPublished - 1995
外部発表はい

ASJC Scopus subject areas

  • 分析化学

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