Direct observation of the myosin va recovery stroke that contributes to unidirectional stepping along actin

Katsuyuki Shiroguchi*, Harvey F. Chin, Diane E. Hannemann, Eiro Muneyuki, Enrique M. de la Cruz, Kazuhiko Kinosita

*この研究の対応する著者

    研究成果査読

    20 被引用数 (Scopus)

    抄録

    Myosins are ATP-driven linear molecular motors that work as cellular force generators, transporters, and force sensors. These functions are driven by large-scale nucleotide-dependent conformational changes, termed "strokes"; the "power stroke" is the force-generating swinging of the myosin light chain-binding "neck" domain relative to the motor domain "head" while bound to actin; the "recovery stroke" is the necessary initial motion that primes, or "cocks," myosin while detached from actin. Myosin Va is a processive dimer that steps unidirectionally along actin following a "hand over hand" mechanism in which the trailing head detaches and steps forward ~72 nm. Despite large rotational Brownian motion of the detached head about a free joint adjoining the two necks, unidirectional stepping is achieved, in part by the power stroke of the attached head that moves the joint forward. However, the power stroke alone cannot fully account for preferential forward site binding since the orientation and angle stability of the detached head, which is determined by the properties of the recovery stroke, dictate actin binding site accessibility. Here, we directly observe the recovery stroke dynamics and fluctuations of myosin Va using a novel, transient caged ATP-controlling system that maintains constant ATP levels through stepwise UV-pulse sequences of varying intensity. We immobilized the neck of monomeric myosin Va on a surface and observed real time motions of bead(s) attached site-specifically to the head. ATP induces a transient swing of the neck to the post-recovery stroke conformation, where it remains for ∼40 s, until ATP hydrolysis products are released. Angle distributions indicate that the post-recovery stroke conformation is stabilized by ≥5 kBT of energy. The high kinetic and energetic stability of the post-recovery stroke conformation favors preferential binding of the detached head to a forward site 72 nm away. Thus, the recovery stroke contributes to unidirectional stepping of myosin Va.

    本文言語English
    論文番号e1001031
    ジャーナルPLoS Biology
    9
    4
    DOI
    出版ステータスPublished - 2011 4

    ASJC Scopus subject areas

    • 農業および生物科学(全般)
    • 生化学、遺伝学、分子生物学(全般)
    • 免疫学および微生物学(全般)
    • 神経科学(全般)

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