Direct organelle thermometry with fluorescence lifetime imaging microscopy in single myotubes

Hideki Itoh, Satoshi Arai, Thankiah Sudhaharan, Sung Chan Lee, Young Tae Chang, Shin'ichi Ishiwata, Madoka Suzuki, E. Birgitte Lane

研究成果: Article

22 引用 (Scopus)

抜粋

We describe organelle thermometry using an endoplasmic reticulum-targeting small molecule dye and cytosolic mCherry, whose fluorescence lifetimes reduce with increasing temperature and can be monitored by fluorescence lifetime imaging microscopy. The results show that heat production in single myotubes is highly localized and is coupled to a Ca2+ burst.

元の言語English
ページ(範囲)4458-4461
ページ数4
ジャーナルChemical Communications
52
発行部数24
DOI
出版物ステータスPublished - 2016

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Ceramics and Composites
  • Electronic, Optical and Magnetic Materials
  • Surfaces, Coatings and Films
  • Materials Chemistry
  • Metals and Alloys

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  • これを引用

    Itoh, H., Arai, S., Sudhaharan, T., Lee, S. C., Chang, Y. T., Ishiwata, S., Suzuki, M., & Lane, E. B. (2016). Direct organelle thermometry with fluorescence lifetime imaging microscopy in single myotubes. Chemical Communications, 52(24), 4458-4461. https://doi.org/10.1039/c5cc09943a