Directed evolution of carotenoid synthases for the production of unnatural carotenoids

Maiko Furubayashi, Daisuke Umeno

研究成果: Chapter

8 被引用数 (Scopus)

抄録

Directed evolution is a well-established strategy to confer novel catalytic functions to the enzymes. Thanks to the relative ease of establishing color screening, carotenogenic enzymes can be rapidly evolved in the laboratory for novel functions. The combinatorial usages of the evolvants result in the creation of diverse set of novel, sometimes unnatural carotenoids. This chapter describes the directed evolution of diapophytoene (C30 carotenoid) synthase CrtM to function in the foreign C40 pathway, and the use of the CrtM variants thus obtained for the production of novel backbone structures.

本文言語English
ホスト出版物のタイトルMicrobial Carotenoids from Bacteria and Microalgae
ホスト出版物のサブタイトルMethods and Protocols
出版社Humana Press Inc.
ページ245-253
ページ数9
ISBN(印刷版)9781617798788
DOI
出版ステータスPublished - 2012
外部発表はい

出版物シリーズ

名前Methods in Molecular Biology
892
ISSN(印刷版)1064-3745

ASJC Scopus subject areas

  • 分子生物学
  • 遺伝学

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