Directed evolution of squalene synthase for dehydrosqualene biosynthesis

Maiko Furubayashi, Ling Li, Akinori Katabami, Kyoichi Saito, Daisuke Umeno*

*この研究の対応する著者

研究成果: Article査読

9 被引用数 (Scopus)

抄録

Squalene synthase (SQS) catalyzes the first step of sterol/hopanoid biosynthesis in various organisms. It has been long recognized that SQSs share a common ancestor with carotenoid synthases, but it is not known how these enzymes selectively produce their own product. In this study, SQSs from yeast, human, and bacteria were independently subjected to directed evolution for the production of the C30 carotenoid backbone, dehydrosqualene. This was accomplished via high-throughput screening with Pantoea ananatis phytoene desaturase, which can selectively convert dehydrosqualene into yellow carotenoid pigments. Genetic analysis of the resultant mutants revealed various mutations that could effectively convert SQS into a "dehydrosqualene synthase." All of these mutations are clustered around the residues that have been proposed to be important for NADPH binding.

本文言語English
ページ(範囲)3375-3381
ページ数7
ジャーナルFEBS Letters
588
18
DOI
出版ステータスPublished - 2014 9 17
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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