Discontinuous total parenteral nutrition prevents postischemic mitochondrial dysfunction in rat liver

Nobuyuki Morikawa, Makoto Suematsu*, Takanori Kyokane, Nobuhito Goda, Yusuke Kumamoto, Taro Okitsu, Yuzuru Ishimura, Masaki Kitajima

*この研究の対応する著者

研究成果: Article査読

15 被引用数 (Scopus)

抄録

Although discontinuous total parenteral nutrition (d-TPN) has recently been favored for clinical use over continuous total parenteral nutrition (c- TPN) to ameliorate liver dysfunction, mechanisms for the protection against postoperative liver dysfunction remain unknown. This study aimed to examine differences in mitochondrial function in d-TPN-and c-TPN-pretreated livers during ischemia-reperfusion. Rat livers pretreated with d-TPN or c-TPN were perfused with Krebs-Ringer buffer and were exposed to 25% low-flow hypoxia followed by reperfusion. Intrahepatic mitochondrial membrane potential (ΔΨ) and cell viability were assessed by dual-color digital microfluorography using rhodamine 123 (Rh123) and propidium iodide (PI), respectively. In response to hypoxia, livers pretreated with c-TPN, d-TPN, and an ordinary chow diet exhibited a significant ΔΨ reduction among the entire lobules. Upon reperfusion, the regional ΔΨ values further decreased in the c-TPN liver, whereas those in the d-TPN-treated or chow-treated livers displayed a rapid recovery toward the control levels. The severity of cell injury did not differ among the groups, showing that the reperfusion-induced ΔΨ drop in the c-TPN-pretreated liver is not a consequence of cell injury. Differences in the ΔΨ drop among the groups appear to occur irrespective of those in the glycogen storage, because the livers undergoing d-TPN display a marked ΔΨ recovery even when reperfused at the end of a fasted state. These results indicate that c-TPN, but not d-TPN, jeopardizes mitochondrial re- energization and suggest that a circadian pattern of the TPN serves as a potentially beneficial strategy to reduce the risk of postischemic mitochondrial dysfunction in the liver.

本文言語English
ページ(範囲)1289-1299
ページ数11
ジャーナルHepatology
28
5
DOI
出版ステータスPublished - 1998 11月 10
外部発表はい

ASJC Scopus subject areas

  • 肝臓学

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