Discovery of 2-naphthoic acid monooxygenases by genome mining and their use as biocatalysts

Toshiki Furuya, Kuniki Kino

研究成果: Article査読

26 被引用数 (Scopus)

抄録

The large pool of cytochrome P450 (P450) open-reading frames identified in genome sequences has attracted much attention as a resource for new oxidation biocatalysts. P450 genes were cloned from genome-sequenced bacteria and coexpressed with putidaredoxin and its reductase genes to provide the redox partners of P450 in Escherichia coli. Wholecell assays were performed with 2-naphthoic acid as a substrate. Hydroxylated naphthoic acid products were rapidly detected with two reagents showing different colors in the presence of the products. Two P450s, CYP199A1 and CYP199A2, were found to hydroxylate the substrate to 7-and 8-hydroxy-2-naphthoic acids. The CYP199A1 whole-cell biocatalyst converted 1 mm 2-naphthoic acid to 0.27 mm 7-hydroxy-2-naphthoic acid and 0.53 mm 8-hydroxy-2-naphthoic acid. CYP199A2 exhibited similar regioselectivity to CYP199A1. Furthermore, we found that 8-hydroxy-2-naphthoic acid emits near-white fluorescence when exposed to UV light. These P450s will provide a facile and environmentally friendly synthetic approach to the hydroxynaphthoic acids.

本文言語English
ページ(範囲)645-649
ページ数5
ジャーナルChemSusChem
2
7
DOI
出版ステータスPublished - 2009 7

ASJC Scopus subject areas

  • 環境化学
  • 化学工学(全般)
  • 材料科学(全般)
  • エネルギー(全般)

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