Display of Functionally Active PHB Depolymerase on Escherichia Coli Cell Surface

Tomohiro Hiraishi, Koichi Yamashita, Masafumi Sakono, Jun Nakanishi, Liu Tzea Tan, Kumar Sudesh, Hideki Abe, Mizuo Maeda

研究成果: Article査読

5 被引用数 (Scopus)

抄録

The display of PHB depolymerase (PhaZ RpiT1) from R. pickettii T1 on the surface of E. coli JM109 cells is realized using OprI of P. aeruginosa as the anchoring motif. The fusion protein is stably expressed and its surface localization is verified by immunofluorescence microscopy. The displayed PhaZ RpiT1 retains its cleaving ability for soluble substrates as well as its ability to adsorb to the PHB surface, and also remains catalycically active in the degradation of insoluble polyester materials, in spite of the possible suppression of the enzyme movement on the polymer surface. The results demonstrate that PhaZ RpiT1-displaying E. coli shows potential for use as a whole-cell biocatalyst for the production of (R)-3-hydroxybutyrate monomers from insoluble PHB materials.

本文言語English
ページ(範囲)218-224
ページ数7
ジャーナルMacromolecular Bioscience
12
2
DOI
出版ステータスPublished - 2012 2
外部発表はい

ASJC Scopus subject areas

  • バイオテクノロジー
  • バイオエンジニアリング
  • 生体材料
  • ポリマーおよびプラスチック
  • 材料化学

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