Diurnal regulation of a DNA binding protein to the period repeat sequence in the SCN nuclear extract of rat brain

Toshiyuki Hamada, Koichiro Kako, Hisanori Wakamatsu, Shigenobu Shibata, Shigenori Watanabe, Norio Ishida

研究成果: Article査読

1 被引用数 (Scopus)

抄録

We previously reported the mammalian period repeat mRNA fluctuates during circadian time in the rat suprachiasmatic nucleus (SCN) which is considered to be a clock pacemaker in mammalian brain. Presently we discovered a period repeat sequence (PR) DNA-binding protein in the rat SCN nuclear extract. In the SCN, the binding activity of PR DNA-binding protein to (ACAGGC)3 was most highest during the late day and most lowest during the late night by electro-mobility shift assay (EMSA). In the cortex nuclear extract, the binding of PR DNA-binding protein did not show a significant variation during a day. This is the first report to show the existence of diurnal regulated PR DNA-binding protein in the SCN.

本文言語English
ページ(範囲)211-215
ページ数5
ジャーナルMolecular Brain Research
65
2
DOI
出版ステータスPublished - 1999 3 5

ASJC Scopus subject areas

  • 分子生物学
  • 細胞および分子神経科学

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