DNA conformation of the region preceding the beta-lactamase promoter of pUC19 which is required for efficient transcription.

Y. Hirota, T. Ohyama

研究成果: Article

1 引用 (Scopus)

抜粋

The upstream region of the beta-lactamase promoter of Escherichia coli plasmid pUC19 has a DNA curvature (bent DNA). This region was replaced with another sequence by using randomly synthesized oligonucleotides. Among the reconstructed plasmids, a plasmid which could endow E. coli cells with the strongest resistance to ampicillin on plates was selected. Nucleotide sequence and DNA conformation of the altered region was investigated.

元の言語English
ページ(範囲)151-152
ページ数2
ジャーナルNucleic acids symposium series
発行部数27
出版物ステータスPublished - 1992
外部発表Yes

ASJC Scopus subject areas

  • Medicine(all)

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