DNA methylation-mediated control of Sry gene expression in mouse gonadal development

Koichiro Nishino, Naoko Hattori, Satoshi Tanaka, Kunio Shiota

研究成果: Article

113 引用 (Scopus)

抄録

DNA methylation at CpG sequences is involved in tissue-specific and developmentally regulated gene expression. The Sry (sex-determining region on the Y chromosome) gene encodes a master protein for initiating testis differentiation in mammals, and its expression is restricted to gonadal somatic cells at 10.5-12.5 days postcoitum (dpe) in the mouse. We found that in vitro methylation of the 5′-flanking region of the Sry gene caused suppression of reporter activity, implying that Sry gene expression could be regulated by DNA methylation-mediated gene silencing. Bisulfite restriction mapping and sodium bisulfite sequencing revealed that the 5′-flanking region of the Sry gene was hypermethylated in the 8.5-dpc embryos in which the Sry gene was not expressed. Importantly, this region was specifically hypomethylated in the gonad at 11.5 dpc, while the hypermethylated status was maintained in tissues that do not express the Sry gene. We concluded that expression of the Sry gene is under the control of an epigenetic mechanism mediated by DNA methylation.

元の言語English
ページ(範囲)22306-22313
ページ数8
ジャーナルJournal of Biological Chemistry
279
発行部数21
DOI
出版物ステータスPublished - 2004 5 21
外部発表Yes

Fingerprint

sry Genes
DNA Methylation
Gene expression
Genes
Gene Expression
5' Flanking Region
Y-Linked Genes
Restriction Mapping
Tissue
Gonads
Gene Silencing
Methylation
Mammals
Epigenomics
Chromosomes
Testis
Embryonic Structures

ASJC Scopus subject areas

  • Biochemistry

これを引用

DNA methylation-mediated control of Sry gene expression in mouse gonadal development. / Nishino, Koichiro; Hattori, Naoko; Tanaka, Satoshi; Shiota, Kunio.

:: Journal of Biological Chemistry, 巻 279, 番号 21, 21.05.2004, p. 22306-22313.

研究成果: Article

Nishino, Koichiro ; Hattori, Naoko ; Tanaka, Satoshi ; Shiota, Kunio. / DNA methylation-mediated control of Sry gene expression in mouse gonadal development. :: Journal of Biological Chemistry. 2004 ; 巻 279, 番号 21. pp. 22306-22313.
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