DNA microarray analysis of redox-responsive genes in the genome of the cyanobacterium Synechocystis sp. strain PCC 6803

Yukako Hihara, Kintake Sonoike, Minoru Kanehisa, Masahiko Ikeuchi

研究成果: Article査読

130 被引用数 (Scopus)

抄録

Whole-genome DNA microarrays were used to evaluate the effect of the redox state of the photosynthetic electron transport chain on gene expression in Synechocystis sp. strain PCC 6803. Two specific inhibitors of electron transport, 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) and 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone (DBMIB), were added to the cultures, and changes in accumulation of transcripts were examined. About 140 genes were highlighted as reproducibly affected by the change in the redox state of the photosynthetic electron transport chain. It was shown that some stress-responsive genes but not photosynthetic genes were under the control of the redox state of the plastoquinone pool in Synechocystis sp. strain PCC 6803.

本文言語English
ページ(範囲)1719-1725
ページ数7
ジャーナルJournal of Bacteriology
185
5
DOI
出版ステータスPublished - 2003 3
外部発表はい

ASJC Scopus subject areas

  • 微生物学
  • 分子生物学

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