DNA sequence motifs are associated with aberrant homologous recombination in the mouse macrophage migration inhibitory factor (Mif) locus

Shigetoshi Kobayashi, Kunihiro Yoshida, Toshio Ohshima, Noriko Esumi, Vishwas M. Paralkar, Graeme J. Wistow, Ashok B. Kulkarni

研究成果: Article

4 引用 (Scopus)

抄録

Homologous recombination is a precise genetic event that can introduce specific alteration in the genome. A planned targeted disruption by homologous recombination of the macrophage migration inhibitory factor (Mif) locus in mouse embryonic stem (ES) cells yielded the targeted clones, some of which had genomic rearrangements inconsistent with the expected homologous recombination event. A detailed characterization of the recombination breakpoints in two of these clones revealed several sequence motifs with possible roles in recombination. These motifs included short regions of sequence identity that may promote DNA alignment, multiple 5'-AAGG/TTCC-3' tetrameres, topoisomerase I consensus sites, and AT-rich sequences that can promote DNA cleavage and recombination. A retrovirus-like intracisternal-A particle (IAP) family sequence was also identified upstream of the Mif gene, and the LTR of this IAP was involved in one of the recombinations. Identification and characterization of such sequence motifs will be valuable for the gene targeting experiments.

元の言語English
ページ(範囲)85-92
ページ数8
ジャーナルGene
215
発行部数1
DOI
出版物ステータスPublished - 1998 7 17
外部発表Yes

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Nucleotide Motifs
Homologous Recombination
Genetic Recombination
Intracisternal A-Particle Genes
Spiruroidea
Clone Cells
AT Rich Sequence
Macrophage Migration-Inhibitory Factors
Type I DNA Topoisomerase
DNA Cleavage
Gene Targeting
Retroviridae
Genome
Mouse Mif protein
DNA

ASJC Scopus subject areas

  • Genetics

これを引用

DNA sequence motifs are associated with aberrant homologous recombination in the mouse macrophage migration inhibitory factor (Mif) locus. / Kobayashi, Shigetoshi; Yoshida, Kunihiro; Ohshima, Toshio; Esumi, Noriko; Paralkar, Vishwas M.; Wistow, Graeme J.; Kulkarni, Ashok B.

:: Gene, 巻 215, 番号 1, 17.07.1998, p. 85-92.

研究成果: Article

Kobayashi, Shigetoshi ; Yoshida, Kunihiro ; Ohshima, Toshio ; Esumi, Noriko ; Paralkar, Vishwas M. ; Wistow, Graeme J. ; Kulkarni, Ashok B. / DNA sequence motifs are associated with aberrant homologous recombination in the mouse macrophage migration inhibitory factor (Mif) locus. :: Gene. 1998 ; 巻 215, 番号 1. pp. 85-92.
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AU - Yoshida, Kunihiro

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AU - Esumi, Noriko

AU - Paralkar, Vishwas M.

AU - Wistow, Graeme J.

AU - Kulkarni, Ashok B.

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AB - Homologous recombination is a precise genetic event that can introduce specific alteration in the genome. A planned targeted disruption by homologous recombination of the macrophage migration inhibitory factor (Mif) locus in mouse embryonic stem (ES) cells yielded the targeted clones, some of which had genomic rearrangements inconsistent with the expected homologous recombination event. A detailed characterization of the recombination breakpoints in two of these clones revealed several sequence motifs with possible roles in recombination. These motifs included short regions of sequence identity that may promote DNA alignment, multiple 5'-AAGG/TTCC-3' tetrameres, topoisomerase I consensus sites, and AT-rich sequences that can promote DNA cleavage and recombination. A retrovirus-like intracisternal-A particle (IAP) family sequence was also identified upstream of the Mif gene, and the LTR of this IAP was involved in one of the recombinations. Identification and characterization of such sequence motifs will be valuable for the gene targeting experiments.

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