Down-regulation of human galβ1,3GalNAc/galβ1,4GlcNAc α2,3-sialyltransferase (hST3Gal IV) gene during differentiation of the HL-60 cell line

Akiyoshi Taniguchi, Yuko Hasegawa, Koji Higai, Kojiro Matsumoto

研究成果: Article

12 被引用数 (Scopus)

抄録

We studied, the regulation of the human Galβ1, 3GalNAc/Galβ1,4GlcNAc α2,3-sialyltransferase (hST3Gal IV) gene during HL-60 cell differentiation induced by dimethyl sulfoxide (DMSO), all trans-retinoic acid (ATRA), and phorbol myristate acetate (PMA). During differentiation, levels of hST3Gal IV mRNA dramatically decreased after 1 day of stimulation. Reversetranscription PCR identified two mRNA isoforms, types B1 and BX, in HL-60 cells. The results of luciferase assays showed that the level of B3 promoter activity is high, whereas A1/2 and B2 promoter activities are low in HL-60 cells, suggesting that type B1, BX, and B3 mRNA isoforms are expressed in HL-60 cells. A luciferase assay identified a functional DNA portion within the proximal region of the B3 promoter that confers negative transcriptional regulation on the hST3Gal IV B3 promoter during HL-60 differentiation. These results suggest that this element plays a critical role in down-regulating the B3 promoter activity during HL-60 cell differentiation.

本文言語English
ページ(範囲)197-201
ページ数5
ジャーナルMolecular Cell Biology Research Communications
2
3
DOI
出版ステータスPublished - 1999 9
外部発表はい

ASJC Scopus subject areas

  • Molecular Biology

フィンガープリント 「Down-regulation of human galβ1,3GalNAc/galβ1,4GlcNAc α2,3-sialyltransferase (hST3Gal IV) gene during differentiation of the HL-60 cell line」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

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