Downregulation of an AIM-1 kinase couples with megakaryocytic polyploidization of human hematopoietic cells

Akira Kawasaki, Itaru Matsumura, Jun Ichiro Miyagawa, Sachiko Ezoe, Hirokazu Tanaka, Yasuhiko Terada, Masaaki Tatsuka, Takashi Machii, Hiroshi Miyazaki, Yusuke Furukawa, Yuzuru Kanakura

研究成果: Article

53 引用 (Scopus)

抄録

During the late phase of megakaryopoiesis, megakaryocytes undergo polyploidization, which is characterized by DNA duplication without concomitant cell division. However, it remains unknown by which mechanisms this process occurs. AIM-1 and STK15 belong to the Aurora/increase-in-ploidy (Ipl)1 serine/threonine kinase family and play key roles in mitosis. In a human interleukin-3-dependent cell line, F-36P, the expressions of AIM-1 and STK15 mRNA were specifically observed at G2/M phase of the cell cycle during proliferation. In contrast, the expressions of AIM-1 and STK15 were continuously repressed during megakaryocytic polyploidization of human erythro/megakaryocytic cell lines (F-36P, K562, and CMK) treated with thrombopoietin, activated ras (H-rasG12V), or phorbol ester. Furthermore, their expressions were suppressed during thrombopoietin-induced polyploidization of normal human megakaryocytes. Activation of AIM-1 by the induced expression of AIM-1(wild-type) canceled TPA-induced polyploidization of K562 cells significantly, whereas that of STK15 did not. Moreover, suppression of AIM-1 by the induced expression of AIM-1 (K/R, dominant-negative type) led to polyploidization in 25% of K562 cells, whereas STK15(K/R) showed no effect. Also, the induced expression of AIM-1(k/R) in CMK cells provoked polyploidization up to 32N. These results suggested that downregulation of AIM-1 at M phase may be involved in abortive mitosis and polyploid formation of megakaryocytes.

元の言語English
ページ(範囲)275-287
ページ数13
ジャーナルJournal of Cell Biology
152
発行部数2
DOI
出版物ステータスPublished - 2001 1 22
外部発表Yes

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Megakaryocytes
Cell Division
Thrombopoietin
Phosphotransferases
Down-Regulation
K562 Cells
Mitosis
Cell Line
Polyploidy
Ploidies
Interleukin-3
G2 Phase
Protein-Serine-Threonine Kinases
Phorbol Esters
Cell Cycle
Messenger RNA
DNA

ASJC Scopus subject areas

  • Cell Biology

これを引用

Downregulation of an AIM-1 kinase couples with megakaryocytic polyploidization of human hematopoietic cells. / Kawasaki, Akira; Matsumura, Itaru; Miyagawa, Jun Ichiro; Ezoe, Sachiko; Tanaka, Hirokazu; Terada, Yasuhiko; Tatsuka, Masaaki; Machii, Takashi; Miyazaki, Hiroshi; Furukawa, Yusuke; Kanakura, Yuzuru.

:: Journal of Cell Biology, 巻 152, 番号 2, 22.01.2001, p. 275-287.

研究成果: Article

Kawasaki, A, Matsumura, I, Miyagawa, JI, Ezoe, S, Tanaka, H, Terada, Y, Tatsuka, M, Machii, T, Miyazaki, H, Furukawa, Y & Kanakura, Y 2001, 'Downregulation of an AIM-1 kinase couples with megakaryocytic polyploidization of human hematopoietic cells', Journal of Cell Biology, 巻. 152, 番号 2, pp. 275-287. https://doi.org/10.1083/jcb.152.2.275
Kawasaki, Akira ; Matsumura, Itaru ; Miyagawa, Jun Ichiro ; Ezoe, Sachiko ; Tanaka, Hirokazu ; Terada, Yasuhiko ; Tatsuka, Masaaki ; Machii, Takashi ; Miyazaki, Hiroshi ; Furukawa, Yusuke ; Kanakura, Yuzuru. / Downregulation of an AIM-1 kinase couples with megakaryocytic polyploidization of human hematopoietic cells. :: Journal of Cell Biology. 2001 ; 巻 152, 番号 2. pp. 275-287.
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abstract = "During the late phase of megakaryopoiesis, megakaryocytes undergo polyploidization, which is characterized by DNA duplication without concomitant cell division. However, it remains unknown by which mechanisms this process occurs. AIM-1 and STK15 belong to the Aurora/increase-in-ploidy (Ipl)1 serine/threonine kinase family and play key roles in mitosis. In a human interleukin-3-dependent cell line, F-36P, the expressions of AIM-1 and STK15 mRNA were specifically observed at G2/M phase of the cell cycle during proliferation. In contrast, the expressions of AIM-1 and STK15 were continuously repressed during megakaryocytic polyploidization of human erythro/megakaryocytic cell lines (F-36P, K562, and CMK) treated with thrombopoietin, activated ras (H-rasG12V), or phorbol ester. Furthermore, their expressions were suppressed during thrombopoietin-induced polyploidization of normal human megakaryocytes. Activation of AIM-1 by the induced expression of AIM-1(wild-type) canceled TPA-induced polyploidization of K562 cells significantly, whereas that of STK15 did not. Moreover, suppression of AIM-1 by the induced expression of AIM-1 (K/R, dominant-negative type) led to polyploidization in 25{\%} of K562 cells, whereas STK15(K/R) showed no effect. Also, the induced expression of AIM-1(k/R) in CMK cells provoked polyploidization up to 32N. These results suggested that downregulation of AIM-1 at M phase may be involved in abortive mitosis and polyploid formation of megakaryocytes.",
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T1 - Downregulation of an AIM-1 kinase couples with megakaryocytic polyploidization of human hematopoietic cells

AU - Kawasaki, Akira

AU - Matsumura, Itaru

AU - Miyagawa, Jun Ichiro

AU - Ezoe, Sachiko

AU - Tanaka, Hirokazu

AU - Terada, Yasuhiko

AU - Tatsuka, Masaaki

AU - Machii, Takashi

AU - Miyazaki, Hiroshi

AU - Furukawa, Yusuke

AU - Kanakura, Yuzuru

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N2 - During the late phase of megakaryopoiesis, megakaryocytes undergo polyploidization, which is characterized by DNA duplication without concomitant cell division. However, it remains unknown by which mechanisms this process occurs. AIM-1 and STK15 belong to the Aurora/increase-in-ploidy (Ipl)1 serine/threonine kinase family and play key roles in mitosis. In a human interleukin-3-dependent cell line, F-36P, the expressions of AIM-1 and STK15 mRNA were specifically observed at G2/M phase of the cell cycle during proliferation. In contrast, the expressions of AIM-1 and STK15 were continuously repressed during megakaryocytic polyploidization of human erythro/megakaryocytic cell lines (F-36P, K562, and CMK) treated with thrombopoietin, activated ras (H-rasG12V), or phorbol ester. Furthermore, their expressions were suppressed during thrombopoietin-induced polyploidization of normal human megakaryocytes. Activation of AIM-1 by the induced expression of AIM-1(wild-type) canceled TPA-induced polyploidization of K562 cells significantly, whereas that of STK15 did not. Moreover, suppression of AIM-1 by the induced expression of AIM-1 (K/R, dominant-negative type) led to polyploidization in 25% of K562 cells, whereas STK15(K/R) showed no effect. Also, the induced expression of AIM-1(k/R) in CMK cells provoked polyploidization up to 32N. These results suggested that downregulation of AIM-1 at M phase may be involved in abortive mitosis and polyploid formation of megakaryocytes.

AB - During the late phase of megakaryopoiesis, megakaryocytes undergo polyploidization, which is characterized by DNA duplication without concomitant cell division. However, it remains unknown by which mechanisms this process occurs. AIM-1 and STK15 belong to the Aurora/increase-in-ploidy (Ipl)1 serine/threonine kinase family and play key roles in mitosis. In a human interleukin-3-dependent cell line, F-36P, the expressions of AIM-1 and STK15 mRNA were specifically observed at G2/M phase of the cell cycle during proliferation. In contrast, the expressions of AIM-1 and STK15 were continuously repressed during megakaryocytic polyploidization of human erythro/megakaryocytic cell lines (F-36P, K562, and CMK) treated with thrombopoietin, activated ras (H-rasG12V), or phorbol ester. Furthermore, their expressions were suppressed during thrombopoietin-induced polyploidization of normal human megakaryocytes. Activation of AIM-1 by the induced expression of AIM-1(wild-type) canceled TPA-induced polyploidization of K562 cells significantly, whereas that of STK15 did not. Moreover, suppression of AIM-1 by the induced expression of AIM-1 (K/R, dominant-negative type) led to polyploidization in 25% of K562 cells, whereas STK15(K/R) showed no effect. Also, the induced expression of AIM-1(k/R) in CMK cells provoked polyploidization up to 32N. These results suggested that downregulation of AIM-1 at M phase may be involved in abortive mitosis and polyploid formation of megakaryocytes.

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