Dpysl2 (Crmp2) is required for the migration of facial branchiomotor neurons in the developing zebrafish embryo

Carolina Fiallos-Oliveros, Toshio Ohshima*

*この研究の対応する著者

研究成果: Article査読

抄録

Dihydropyrimidinase-like family proteins (Dpysls) are relevant in several processes during nervous system development; among others, they are involved in axonal growth and cell migration. Dpysl2 (CRMP2) is the most studied member of this family; however, its role in vivo is still being investigated. Our previous studies in zebrafish showed the requirement of Dpysl2 for the proper positioning of caudal primary motor neurons and Rohon-Beard neurons in the spinal cord. In the present study, we show that Dpysl2 is necessary for the proper migration of facial bran-chiomotor neurons during early development in zebrafish. We generated a dpysl2 knock-out (KO) zebrafish mutant line and used different types of antisense morpholino oligonucleotides (AMO) to analyze the role of Dpysl2 in this process. Both dpysl2 KO mutants and morphants exhibited abnormalities in the migration of these neurons from rhombomers (r) 4 and 5 to 6 and 7. The facial branchiomotor neurons that were expected to be at r6 were still located at r4 and r5 hours after the migration process should have been completed. In addition, mutant phenotypes were rescued by injecting dpysl2 mRNA into the KO embryos. These results indicate that Dpysl2 is involved in the proper migration of facial branchiomotor neurons in developing zebrafish embryos.

本文言語English
ページ(範囲)479-484
ページ数6
ジャーナルInternational Journal of Developmental Biology
64
11-12
DOI
出版ステータスPublished - 2020

ASJC Scopus subject areas

  • 胎生学
  • 発生生物学

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