Droplet microfluidics for precise and high throughput whole genome amplification toward single-cell genome sequencing

Masahito Hosokawa, Y. Nishikawa, Masato Kogawa, Haruko Takeyama

研究成果: Conference contribution

抜粋

Single-cell genomics has enabled the exploration of cellular diversity in a broad range of biological samples including tumor and environmental microbes. However, next-generation sequencing (NGS) typically requires nanogram to microgram levels of input DNA. Since single cells contain only a few femtograms (microbe) to picograms (mammalian) of DNA, whole genome amplification (WGA) is required to amplify single-cell genome to adequate amount for sequencing. In addition, high-throughput genome amplification is required to obtain various data from heterogeneous cell populations. To address these problems, we aimed to develop novel system for precise and high throughput single-cell genomics, to elucidate environmental microbial diversity. In this study, we have developed droplet-based microfluidic system to produce the compartmentalized reaction environments for single-cell whole genome amplification.

元の言語English
ホスト出版物のタイトル20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
出版者Chemical and Biological Microsystems Society
ページ178-179
ページ数2
ISBN(電子版)9780979806490
出版物ステータスPublished - 2016
イベント20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
継続期間: 2016 10 92016 10 13

Other

Other20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Ireland
Dublin
期間16/10/916/10/13

ASJC Scopus subject areas

  • Control and Systems Engineering

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  • これを引用

    Hosokawa, M., Nishikawa, Y., Kogawa, M., & Takeyama, H. (2016). Droplet microfluidics for precise and high throughput whole genome amplification toward single-cell genome sequencing. : 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (pp. 178-179). Chemical and Biological Microsystems Society.