Drosophila tRNAs hyperprocessed in vitro by ribonuclease P.

Y. Hori, S. Hashida, M. Koike, T. Tanaka, Yo Kikuchi

研究成果: Article

抄録

In transposon copia-related retrovirus-like particles of Drosophila, a 5' half fragment produced by the cleavage of mature initiator methionine tRNA is used as the primer for minus-strand reverse transcription. This cleavage is called hyperprocessing. We have previously reported that the catalytic RNA subunit of RNase P catalyzes this hyperprocessing in vitro and that this cleavage is dependent on the occurrence of an altered conformation of the tRNA substrate. Here, we found that other mature tRNAs of Drosophila were also hyperprocessed by M1 RNA in vitro and that some of such tRNAs were probably alanine and histidine tRNAs. Here we report these two tRNAs can also adopt their alternative conformations very similar to that of initiator methionine tRNA.

元の言語English
ページ(範囲)259-260
ページ数2
ジャーナルNucleic acids symposium series
発行部数42
出版物ステータスPublished - 1999
外部発表Yes

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Ribonuclease P
Transfer RNA
Drosophila
RNA, Transfer, Met
Methionine
Catalytic RNA
Retroviridae
Histidine
Alanine
Reverse Transcription
Catalytic Domain
In Vitro Techniques
RNA

これを引用

Hori, Y., Hashida, S., Koike, M., Tanaka, T., & Kikuchi, Y. (1999). Drosophila tRNAs hyperprocessed in vitro by ribonuclease P. Nucleic acids symposium series, (42), 259-260.

Drosophila tRNAs hyperprocessed in vitro by ribonuclease P. / Hori, Y.; Hashida, S.; Koike, M.; Tanaka, T.; Kikuchi, Yo.

:: Nucleic acids symposium series, 番号 42, 1999, p. 259-260.

研究成果: Article

Hori, Y, Hashida, S, Koike, M, Tanaka, T & Kikuchi, Y 1999, 'Drosophila tRNAs hyperprocessed in vitro by ribonuclease P.', Nucleic acids symposium series, 番号 42, pp. 259-260.
Hori Y, Hashida S, Koike M, Tanaka T, Kikuchi Y. Drosophila tRNAs hyperprocessed in vitro by ribonuclease P. Nucleic acids symposium series. 1999;(42):259-260.
Hori, Y. ; Hashida, S. ; Koike, M. ; Tanaka, T. ; Kikuchi, Yo. / Drosophila tRNAs hyperprocessed in vitro by ribonuclease P. :: Nucleic acids symposium series. 1999 ; 番号 42. pp. 259-260.
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