Dynamic imaging of single biomolecular interaction using flow control and TIRFM

T. Arakawa, T. Sameshima, Y. Sato, Y. Sumiyoshi, T. Ueno, Y. Shirasaki, T. Funatsu, Shuichi Shoji

    研究成果: Conference contribution

    抄録

    A rapid multi-reagents switching microvalve system integrated with a total internal reflection fluorescence microscopy (TIRFM) was developed for real time imaging of a single protein behavior. The binding and dissociation process between a chaperonin GroEL and cochaperonin GroES was observed in this TIRFM microfluidic system. For single molecular imaging in this system, biotinylated GroEL (D490C) was immobilized to the glass microchannel surface through streptavidin and biotinylated BSA. A solution including 1 nM IC5-GroES was introduced for 100 ms into the observation area and then washed out with 2 mM ATP buffer solution. Fluorescence spots of IC5-GroES appeared after rapid solution switching, and disappeared several seconds later. As a result, we succeeded in detecting fluorescence signal from single molecules in TIRFM microfluidic system.

    元の言語English
    ホスト出版物のタイトル12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference
    出版者Chemical and Biological Microsystems Society
    ページ296-298
    ページ数3
    出版物ステータスPublished - 2008
    イベント12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA
    継続期間: 2008 10 122008 10 16

    Other

    Other12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
    San Diego, CA
    期間08/10/1208/10/16

    Fingerprint

    Fluorescence microscopy
    Flow control
    Imaging techniques
    Microfluidics
    Fluorescence
    Chaperonins
    Molecular imaging
    Streptavidin
    Switching systems
    Adenosinetriphosphate
    Microchannels
    Buffers
    Adenosine Triphosphate
    Proteins
    Glass
    Molecules

    ASJC Scopus subject areas

    • Chemical Engineering (miscellaneous)
    • Bioengineering

    これを引用

    Arakawa, T., Sameshima, T., Sato, Y., Sumiyoshi, Y., Ueno, T., Shirasaki, Y., ... Shoji, S. (2008). Dynamic imaging of single biomolecular interaction using flow control and TIRFM. : 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference (pp. 296-298). Chemical and Biological Microsystems Society.

    Dynamic imaging of single biomolecular interaction using flow control and TIRFM. / Arakawa, T.; Sameshima, T.; Sato, Y.; Sumiyoshi, Y.; Ueno, T.; Shirasaki, Y.; Funatsu, T.; Shoji, Shuichi.

    12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. Chemical and Biological Microsystems Society, 2008. p. 296-298.

    研究成果: Conference contribution

    Arakawa, T, Sameshima, T, Sato, Y, Sumiyoshi, Y, Ueno, T, Shirasaki, Y, Funatsu, T & Shoji, S 2008, Dynamic imaging of single biomolecular interaction using flow control and TIRFM. : 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. Chemical and Biological Microsystems Society, pp. 296-298, 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, 08/10/12.
    Arakawa T, Sameshima T, Sato Y, Sumiyoshi Y, Ueno T, Shirasaki Y その他. Dynamic imaging of single biomolecular interaction using flow control and TIRFM. : 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. Chemical and Biological Microsystems Society. 2008. p. 296-298
    Arakawa, T. ; Sameshima, T. ; Sato, Y. ; Sumiyoshi, Y. ; Ueno, T. ; Shirasaki, Y. ; Funatsu, T. ; Shoji, Shuichi. / Dynamic imaging of single biomolecular interaction using flow control and TIRFM. 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. Chemical and Biological Microsystems Society, 2008. pp. 296-298
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    AU - Arakawa, T.

    AU - Sameshima, T.

    AU - Sato, Y.

    AU - Sumiyoshi, Y.

    AU - Ueno, T.

    AU - Shirasaki, Y.

    AU - Funatsu, T.

    AU - Shoji, Shuichi

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