@inbook{864daa63900f43c9975fcaa59f8c2812,
title = "Early diagnosis with ultrasensitive ELISA",
abstract = "Accurate, rapid and simple detection methods are required to facilitate early diagnosis of various disorders including infectious and lifestyle diseases as well as cancer. These detection approaches reduce the window of infection, i.e., the period between infection and reliable detection. Optimally, these methods should target protein as an indicator of pathogenic microbes as well as other biomarkers. For example, although nucleic acid is easily detected by polymerase chain reaction (PCR), these markers are also present in dead microbes, and, in the case of mRNA, it is not known whether this target was successfully translated. Accordingly, early diagnostic approaches require the development of ultrasensitive protein detection methods. In this chapter, we introduce an ultrasensitive enzyme-linked immunosorbent assay (ELISA) which combines a traditional sandwich-based immunoassay with thionicotinamide adenine dinucleotide (thio-NAD) cycling. The performance characteristics of this unique approach are reviewed as well as its potential role in providing a novel and ultrasensitive diagnostic tool in the clinical laboratory.",
keywords = "Cancer marker, Infectious disease, Lifestyle disease, Non-amplification nucleic acid detection, Single-cell detection, Ultrasensitive ELISA, Window period",
author = "Etsuro Ito and Kanako Iha and Teruki Yoshimura and Kazunari Nakaishi and Satoshi Watabe",
note = "Funding Information: This research was funded by the A-STEP Program from JST (grant number AS3015096U), the Matching Planner Program from JST (grant number VP29117939087), Waseda University grants for Specific Research Projects (grant numbers 2017A-015 and 2019C-123), and the Precise Measurement Technology Promotion Foundation. E.I. and T.Y. received research funds from TAUNS Laboratories, Inc. K.N. and S.W. are employees of TAUNS Laboratories, Inc. Funding Information: This research was funded by the A-STEP Program from JST (grant number AS3015096U), the Matching Planner Program from JST (grant number VP29117939087), Waseda University grants for Specific Research Projects (grant numbers 2017A-015 and 2019C-123), and the Precise Measurement Technology Promotion Foundation. ",
year = "2020",
doi = "10.1016/bs.acc.2020.06.002",
language = "English",
series = "Advances in Clinical Chemistry",
publisher = "Academic Press Inc.",
booktitle = "Advances in Clinical Chemistry",
address = "United States",
}