Early diagnosis with ultrasensitive ELISA

Etsuro Ito*, Kanako Iha, Teruki Yoshimura, Kazunari Nakaishi, Satoshi Watabe

*この研究の対応する著者

研究成果: Chapter

2 被引用数 (Scopus)

抄録

Accurate, rapid and simple detection methods are required to facilitate early diagnosis of various disorders including infectious and lifestyle diseases as well as cancer. These detection approaches reduce the window of infection, i.e., the period between infection and reliable detection. Optimally, these methods should target protein as an indicator of pathogenic microbes as well as other biomarkers. For example, although nucleic acid is easily detected by polymerase chain reaction (PCR), these markers are also present in dead microbes, and, in the case of mRNA, it is not known whether this target was successfully translated. Accordingly, early diagnostic approaches require the development of ultrasensitive protein detection methods. In this chapter, we introduce an ultrasensitive enzyme-linked immunosorbent assay (ELISA) which combines a traditional sandwich-based immunoassay with thionicotinamide adenine dinucleotide (thio-NAD) cycling. The performance characteristics of this unique approach are reviewed as well as its potential role in providing a novel and ultrasensitive diagnostic tool in the clinical laboratory.

本文言語English
ホスト出版物のタイトルAdvances in Clinical Chemistry
編集者Gregory S. Makowski, Gregory S. Makowski
出版社Academic Press Inc.
ページ121-133
ページ数13
ISBN(印刷版)9780128244159
DOI
出版ステータスPublished - 2021 1

出版物シリーズ

名前Advances in Clinical Chemistry
101
ISSN(印刷版)0065-2423
ISSN(電子版)2162-9471

ASJC Scopus subject areas

  • 化学 (全般)
  • 臨床生化学

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