Effect of ε subunit on the rotation of thermophilic Bacillus F1-ATPase

Masato Tsumuraya; Shou Furuike; Kengo Adachi; Kazuhiko Kinosita; Masasuke Yoshida

doi:10.1016/j.febslet.2009.02.038

Effect of ε subunit on the rotation of thermophilic Bacillus F₁-ATPase

Masato Tsumuraya, Shou Furuike, Kengo Adachi, Kazuhiko Kinosita, Masasuke Yoshida^*

^*この研究の対応する著者

研究成果: Article › 査読

22 被引用数 (Scopus)

抄録

F₁-ATPase is an ATP-driven motor in which γε rotates in the α₃β₃-cylinder. It is attenuated by MgADP inhibition and by the ε subunit in an inhibitory form. The non-inhibitory form of ε subunit of thermophilic Bacillus PS3 F₁-ATPase is stabilized by ATP-binding with micromolar K_d at 25 °C. Here, we show that at [ATP] > 2 μM, ε does not affect rotation of PS3 F₁-ATPase but, at 200 nM ATP, ε prolongs the pause of rotation caused by MgADP inhibition while the frequency of the pause is unchanged. It appears that ε undergoes reversible transition to the inhibitory form at [ATP] below K_d.

本文言語	English
ページ（範囲）	1121-1126
ページ数	6
ジャーナル	FEBS Letters
巻	583
号	7
DOI	https://doi.org/10.1016/j.febslet.2009.02.038
出版ステータス	Published - 2009 4月 2

ASJC Scopus subject areas

生化学
生物理学
細胞生物学
遺伝学
分子生物学
構造生物学

文献へのアクセス

10.1016/j.febslet.2009.02.038

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引用スタイル

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AU - Furuike, Shou

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AU - Yoshida, Masasuke

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