Effect of ε subunit on the rotation of thermophilic Bacillus F1-ATPase

Masato Tsumuraya, Shou Furuike, Kengo Adachi, Kazuhiko Kinosita, Masasuke Yoshida*

*この研究の対応する著者

    研究成果: Article査読

    22 被引用数 (Scopus)

    抄録

    F1-ATPase is an ATP-driven motor in which γε rotates in the α3β3-cylinder. It is attenuated by MgADP inhibition and by the ε subunit in an inhibitory form. The non-inhibitory form of ε subunit of thermophilic Bacillus PS3 F1-ATPase is stabilized by ATP-binding with micromolar Kd at 25 °C. Here, we show that at [ATP] > 2 μM, ε does not affect rotation of PS3 F1-ATPase but, at 200 nM ATP, ε prolongs the pause of rotation caused by MgADP inhibition while the frequency of the pause is unchanged. It appears that ε undergoes reversible transition to the inhibitory form at [ATP] below Kd.

    本文言語English
    ページ(範囲)1121-1126
    ページ数6
    ジャーナルFEBS Letters
    583
    7
    DOI
    出版ステータスPublished - 2009 4月 2

    ASJC Scopus subject areas

    • 生化学
    • 生物理学
    • 細胞生物学
    • 遺伝学
    • 分子生物学
    • 構造生物学

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