Effect of bacterial stress on hydrogen fermentation microflora

Hiroyuki Kashima, Hiroshi Nyunoya, Michiei Oto, Seishu Tojo

研究成果: Conference contribution

抄録

In this study, we investigated effects of bacterial stress treatment on domination of hydrogen producing bacteria. As the bacterial stress treatment to control hydrogen fermentation microflora, we tested heat stress (105°C, 2 h) and freeze-thaw stress (a total of 3 cycles, each including-15°C, 12 h and 4°C, 6 h). The effect of bacterial stress on glucose hydrogen fermentation characteristic was determined by batch test using glass bottles. The results of the batch cultivation indicated that hydrogen fermentation characteristics differ among the stressed microflora. This result suggests that each original seed source has the suitable bacterial stress treatment to increase the hydrogen production. At the same time, we conducted 16S rRNA gene based analysis of hydrogen fermentation microbial community by PCR-minigel-single-strand conformation polymorphism (PCR-minigel-SSCP) method. Clostridium species such as Clostridium butyricum were identified from hydrogen fermentation batch culture.

本文言語English
ホスト出版物のタイトルAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2008, ASABE 2008
出版社American Society of Agricultural and Biological Engineers
ページ3201-3211
ページ数11
ISBN(印刷版)9781605605364
出版ステータスPublished - 2008 1 1
外部発表はい
イベントAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2008 - Providence, RI, United States
継続期間: 2008 6 292008 7 2

出版物シリーズ

名前American Society of Agricultural and Biological Engineers Annual International Meeting 2008, ASABE 2008
5

Other

OtherAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2008
CountryUnited States
CityProvidence, RI
Period08/6/2908/7/2

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

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