Effect of Chloride Ion on the Thermal Decay Process of the Batho Intermediate of Iodopsin at Low Temperature

Yasushi Imamoto, Hideki Kandori, Toshiyuki Okano, Yoshitaka Fukada, Yoshinori Shichida, Tôru Yoshizawa*

*この研究の対応する著者

研究成果: Article査読

39 被引用数 (Scopus)

抄録

The photochemical and the subsequent thermal behaviors or iodopsin (Cl--bound form) and N-iodopsin (iodopsin whose Cl- was replaced by NO3-) in CHAPS-phosphatidylcholine (PC) were studied by low-temperature spectrophotometry. Irradiation of the iodopsin preparation at -185 °C produced a photo-steady-state mixture composed of iodopsin, bathoiodopsin, and isoiodopsin. Bathoiodopsin was thermally reverted to the original iodopsin. These results were almost the same as those reported previously [Yoshizawa, T., & Wald, G.(1967) Nature 214, 566-571] in which iodopsin was extracted with 2% digitonin. Therefore, photochemical and subsequent thermal behaviors or iodopsin were independent of the detergent to solubilize iodopsin. Irradiation of N-iodopsin at -185 °C produced the similar photo-steady-state mixture. However, N-bathoiodopsi was thermally converted to the next intermediate, presumably N-lumiiodopsin. These results suggest that the batho-lumi transition of iodopsin at low temperature is likely to be inhibited by the Cl- bound to the protein moiety of iodopsin, while at room temperature the Cl- bound to iodopsin could be released on the conversion process of batho- to lumiiodopsin.

本文言語English
ページ(範囲)9412-9416
ページ数5
ジャーナルBiochemistry
28
24
DOI
出版ステータスPublished - 1989 1月 1
外部発表はい

ASJC Scopus subject areas

  • 生化学

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