Effect of decaglycerol mono-oleate on chemiluminescence of human neutrophils

Qiang Liu*, Katsuhiko Suzuki, Satoru Kudo, Mutsuo Yamada, Kenji Kowatari, Takashi Umeda, Shigeyuki Nakaji, Kazuo Sugawara

*この研究の対応する著者

研究成果査読

3 被引用数 (Scopus)

抄録

Decaglycerol mono-oleate (DGMO), a type of food additive (a polyglycerol ester of fatty acids), was evaluated for its in vitro effect on reactive oxygen species (ROS) generation by human neutrophils using a luminol-dependent chemiluminescence assay. Neutrophils were isolated by the Histopaque-1077/1119 double density gradient technique. Opsonized zymosan (OZ) and phorbol 12-myristate 13-acetate (PMA) were employed as artificial stimuli. DGMO inhibited the chemiluminescence response of OZ-stimulated neutrophils by 58% (p < 0.01) at a concentration of 0.5 mg/mL. DGMO inhibited the PMA-induced chemiluminescence by 40% (p < 0.01) at a concentration of 0.01 mg/mL. The inhibition phenomena could not be attributed to light absorption by DGMO alone, because the percentage chemiluminescence in the presence of DGMO is lower than the percentage transmittance of DGMO at 425 nm. These results suggest that DGMO downregulates ROS generation by human neutrophils.

本文言語English
ページ(範囲)327-330
ページ数4
ジャーナルLuminescence
14
6
DOI
出版ステータスPublished - 1999
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 化学(その他)

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