Four deletion mutant Dictyostelium myosin II heavy chain genes, MyΔ824-941 (Δ1/ 3S2), MyΔ934-1454 (ΔS2), MyΔ934-1194 (ΔS2-1) and MyΔ1157-1454 (ΔS2-2), were transformed by standard electroporation into mhcA- cells (T-null), a mutant Dictyostelium cell devoid of endogenous myosin II heavy chain gene. The growth, development and formation of fruiting bodies of cells expressing those mutant myosin IIs under suspension culture were investigated by comparison with the wild type cell. The results indicate that internal deletion of myosin II affects the growth and development of Dictyostelium. Furthermore, the longer the length of deletion, the more serious the defect in phenotype.
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