Effect of internal deletion of Myosin II on growth and development of Dictyostelium discoideum

Shi Shu, Taro Q.P. Uyeda, Aixiao Liu, Guoqin Liu, Longfei Yen

研究成果: Article査読

抄録

Four deletion mutant Dictyostelium myosin II heavy chain genes, MyΔ824-941 (Δ1/ 3S2), MyΔ934-1454 (ΔS2), MyΔ934-1194 (ΔS2-1) and MyΔ1157-1454 (ΔS2-2), were transformed by standard electroporation into mhcA- cells (T-null), a mutant Dictyostelium cell devoid of endogenous myosin II heavy chain gene. The growth, development and formation of fruiting bodies of cells expressing those mutant myosin IIs under suspension culture were investigated by comparison with the wild type cell. The results indicate that internal deletion of myosin II affects the growth and development of Dictyostelium. Furthermore, the longer the length of deletion, the more serious the defect in phenotype.

本文言語English
ページ(範囲)811-816
ページ数6
ジャーナルChinese Science Bulletin
44
9
DOI
出版ステータスPublished - 1999 5
外部発表はい

ASJC Scopus subject areas

  • General

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