Effect of long-term shear stress exposure on calcium response and morphology of cultured endothelial cells

Susumu Kudo, Ryuhei Yamaguchi, Kazutoshi Machida, Mariko Ikeda, Kotaro Oka, Kazuo Tanishita

研究成果: Article

2 引用 (Scopus)

抄録

Endothelial cells (ECs) that line the inner surface of blood vessels are continuously exposed to shear stress induced by blood flow, in vivo, and shear stress affects the intracellular calcium ([Ca2+]1), which initiates cellular responses. Although shear-stress induced [Ca2+]1 responses shortly (within 10 min after initiation of flow have been studied, the effects of long-term exposure (24 hr) of shear stress on [Ca2+]1 responses have not. Here, we studied the effect of long-term exposure of shear stress on [Ca2+]1 responses in cultured ECs by using a confocal laser microscope and calcium indicator (Calcium Green-1/ AM). At the initiation of shear stress of 2 Pa (0 hr), 27% of the cells exhibited [Ca2+]1 responses. This percentage gradually decreased with increasing exposure time, reaching about 4% after 24 hr of exposure. These data indicate that long-term shear-stress exposure affects [Ca2+]1 responses in cultured ECs.

元の言語English
ページ(範囲)1226-1233
ページ数8
ジャーナルJSME International Journal, Series C: Mechanical Systems, Machine Elements and Manufacturing
46
発行部数4
出版物ステータスPublished - 2003 12
外部発表Yes

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Endothelial cells
Shear stress
Calcium
Blood vessels
Microscopes
Blood
Lasers

ASJC Scopus subject areas

  • Industrial and Manufacturing Engineering
  • Mechanical Engineering

これを引用

Effect of long-term shear stress exposure on calcium response and morphology of cultured endothelial cells. / Kudo, Susumu; Yamaguchi, Ryuhei; Machida, Kazutoshi; Ikeda, Mariko; Oka, Kotaro; Tanishita, Kazuo.

:: JSME International Journal, Series C: Mechanical Systems, Machine Elements and Manufacturing, 巻 46, 番号 4, 12.2003, p. 1226-1233.

研究成果: Article

Kudo, Susumu ; Yamaguchi, Ryuhei ; Machida, Kazutoshi ; Ikeda, Mariko ; Oka, Kotaro ; Tanishita, Kazuo. / Effect of long-term shear stress exposure on calcium response and morphology of cultured endothelial cells. :: JSME International Journal, Series C: Mechanical Systems, Machine Elements and Manufacturing. 2003 ; 巻 46, 番号 4. pp. 1226-1233.
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AU - Kudo, Susumu

AU - Yamaguchi, Ryuhei

AU - Machida, Kazutoshi

AU - Ikeda, Mariko

AU - Oka, Kotaro

AU - Tanishita, Kazuo

PY - 2003/12

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N2 - Endothelial cells (ECs) that line the inner surface of blood vessels are continuously exposed to shear stress induced by blood flow, in vivo, and shear stress affects the intracellular calcium ([Ca2+]1), which initiates cellular responses. Although shear-stress induced [Ca2+]1 responses shortly (within 10 min after initiation of flow have been studied, the effects of long-term exposure (24 hr) of shear stress on [Ca2+]1 responses have not. Here, we studied the effect of long-term exposure of shear stress on [Ca2+]1 responses in cultured ECs by using a confocal laser microscope and calcium indicator (Calcium Green-1/ AM). At the initiation of shear stress of 2 Pa (0 hr), 27% of the cells exhibited [Ca2+]1 responses. This percentage gradually decreased with increasing exposure time, reaching about 4% after 24 hr of exposure. These data indicate that long-term shear-stress exposure affects [Ca2+]1 responses in cultured ECs.

AB - Endothelial cells (ECs) that line the inner surface of blood vessels are continuously exposed to shear stress induced by blood flow, in vivo, and shear stress affects the intracellular calcium ([Ca2+]1), which initiates cellular responses. Although shear-stress induced [Ca2+]1 responses shortly (within 10 min after initiation of flow have been studied, the effects of long-term exposure (24 hr) of shear stress on [Ca2+]1 responses have not. Here, we studied the effect of long-term exposure of shear stress on [Ca2+]1 responses in cultured ECs by using a confocal laser microscope and calcium indicator (Calcium Green-1/ AM). At the initiation of shear stress of 2 Pa (0 hr), 27% of the cells exhibited [Ca2+]1 responses. This percentage gradually decreased with increasing exposure time, reaching about 4% after 24 hr of exposure. These data indicate that long-term shear-stress exposure affects [Ca2+]1 responses in cultured ECs.

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KW - Bio-fluid mechanics

KW - Biological engineering

KW - Calcium ion

KW - Endothelial cells

KW - Shear flow

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