Effect of shear stress on albumin uptake into cultured endothelial cells

Susumu Kudo*, Kenji Ikezawa, Shinji Matsumura, Mariko Ikeda, Kotaro Oka, Kazuo Tanishita

*この研究の対応する著者

研究成果: Chapter

5 被引用数 (Scopus)

抄録

The endothelial cell monolayer exposed to shear stress by a blood flow in vivo is a principal barrier for macromolecules transport across the vascular wall. Abnormal uptake of macromolecules into endothelial cell monolayer causes several vascular diseases. Therefore we studied the effect of shear stress on macromolecules uptake into cultured endothelial cells. After 48hr. exposure to shear stress. endothelial cells on coverslips were incubated at 37°C for 60 minutes in PBS containing Tetrametylrhodamine Isothiocyanate conjugated Albumin (TRITC-Albumin). Thereafter the uptake of albumin and the shape of endothelial cells were observed by a laser scanning confocal microscope. The uptake of albumin into endothelial cells exposed to shear stress increased for lower shear stress range and decreased for high shear stress.

本文言語English
ホスト出版物のタイトルAmerican Society of Mechanical Engineers, Bioengineering Division (Publication) BED
ページ209-210
ページ数2
33
出版ステータスPublished - 1996
外部発表はい

ASJC Scopus subject areas

  • 工学(全般)

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