Effect of shear stress on microvessel network formation of endothelial cells with in vitro three-dimensional model

Akinori Ueda, Masaki Koga, Mariko Ikeda, Susumu Kudo, Kazuo Tanishita

研究成果: Article

55 引用 (Scopus)

抄録

Shear stress stimulus is expected to enhance angiogenesis, the formation of microvessels. We determined the effect of shear stress stimulus on three-dimensional microvessel formation in vitro. Bovine pulmonary microvascular endothelial cells were seeded onto collagen gels with basic fibroblast growth factor to make a microvessel formation model. We observed this model in detail using phase-contrast microscopy, confocal laser scanning microscopy, and electron microscopy. The results show that cells invaded the collagen gel and reconstructed the tubular structures, containing a clearly defined lumen consisting of multiple cells. The model was placed in a parallel-plate flow chamber. A laminar shear stress of 0.3 Pa was applied to the surfaces of the cells for 48 h. Promotion of microvessel network formation was detectable after ∼10 h in the flow chamber. After 48 h, the length of networks exposed to shear stress was 6.17 (±0.59) times longer than at the initial state, whereas the length of networks not exposed to shear stress was only 3.30 (±0.41) times longer. The number of bifurcations and endpoints increased for networks exposed to shear stress, whereas the number of bifurcations alone increased for networks not exposed to shear stress. These results demonstrate that shear stress applied to the surfaces of endothelial cells on collagen gel promotes the growth of microvessel network formation in the gel and expands the network because of repeated bifurcation and elongation.

元の言語English
ジャーナルAmerican Journal of Physiology - Heart and Circulatory Physiology
287
発行部数3 56-3
DOI
出版物ステータスPublished - 2004 9
外部発表Yes

Fingerprint

Microvessels
Endothelial Cells
Gels
Collagen
Phase-Contrast Microscopy
Fibroblast Growth Factor 2
Confocal Microscopy
Electron Microscopy
In Vitro Techniques
Lung
Growth

ASJC Scopus subject areas

  • Physiology

これを引用

Effect of shear stress on microvessel network formation of endothelial cells with in vitro three-dimensional model. / Ueda, Akinori; Koga, Masaki; Ikeda, Mariko; Kudo, Susumu; Tanishita, Kazuo.

:: American Journal of Physiology - Heart and Circulatory Physiology, 巻 287, 番号 3 56-3, 09.2004.

研究成果: Article

Ueda, Akinori ; Koga, Masaki ; Ikeda, Mariko ; Kudo, Susumu ; Tanishita, Kazuo. / Effect of shear stress on microvessel network formation of endothelial cells with in vitro three-dimensional model. :: American Journal of Physiology - Heart and Circulatory Physiology. 2004 ; 巻 287, 番号 3 56-3.
@article{e3d155fd16da4c418993e609d0d701df,
title = "Effect of shear stress on microvessel network formation of endothelial cells with in vitro three-dimensional model",
abstract = "Shear stress stimulus is expected to enhance angiogenesis, the formation of microvessels. We determined the effect of shear stress stimulus on three-dimensional microvessel formation in vitro. Bovine pulmonary microvascular endothelial cells were seeded onto collagen gels with basic fibroblast growth factor to make a microvessel formation model. We observed this model in detail using phase-contrast microscopy, confocal laser scanning microscopy, and electron microscopy. The results show that cells invaded the collagen gel and reconstructed the tubular structures, containing a clearly defined lumen consisting of multiple cells. The model was placed in a parallel-plate flow chamber. A laminar shear stress of 0.3 Pa was applied to the surfaces of the cells for 48 h. Promotion of microvessel network formation was detectable after ∼10 h in the flow chamber. After 48 h, the length of networks exposed to shear stress was 6.17 (±0.59) times longer than at the initial state, whereas the length of networks not exposed to shear stress was only 3.30 (±0.41) times longer. The number of bifurcations and endpoints increased for networks exposed to shear stress, whereas the number of bifurcations alone increased for networks not exposed to shear stress. These results demonstrate that shear stress applied to the surfaces of endothelial cells on collagen gel promotes the growth of microvessel network formation in the gel and expands the network because of repeated bifurcation and elongation.",
keywords = "Angiogenesis, Blood flow, Capillary, In vitro model, Migration",
author = "Akinori Ueda and Masaki Koga and Mariko Ikeda and Susumu Kudo and Kazuo Tanishita",
year = "2004",
month = "9",
doi = "10.1152/ajpheart.00400.2003",
language = "English",
volume = "287",
journal = "American Journal of Physiology - Heart and Circulatory Physiology",
issn = "0363-6135",
publisher = "American Physiological Society",
number = "3 56-3",

}

TY - JOUR

T1 - Effect of shear stress on microvessel network formation of endothelial cells with in vitro three-dimensional model

AU - Ueda, Akinori

AU - Koga, Masaki

AU - Ikeda, Mariko

AU - Kudo, Susumu

AU - Tanishita, Kazuo

PY - 2004/9

Y1 - 2004/9

N2 - Shear stress stimulus is expected to enhance angiogenesis, the formation of microvessels. We determined the effect of shear stress stimulus on three-dimensional microvessel formation in vitro. Bovine pulmonary microvascular endothelial cells were seeded onto collagen gels with basic fibroblast growth factor to make a microvessel formation model. We observed this model in detail using phase-contrast microscopy, confocal laser scanning microscopy, and electron microscopy. The results show that cells invaded the collagen gel and reconstructed the tubular structures, containing a clearly defined lumen consisting of multiple cells. The model was placed in a parallel-plate flow chamber. A laminar shear stress of 0.3 Pa was applied to the surfaces of the cells for 48 h. Promotion of microvessel network formation was detectable after ∼10 h in the flow chamber. After 48 h, the length of networks exposed to shear stress was 6.17 (±0.59) times longer than at the initial state, whereas the length of networks not exposed to shear stress was only 3.30 (±0.41) times longer. The number of bifurcations and endpoints increased for networks exposed to shear stress, whereas the number of bifurcations alone increased for networks not exposed to shear stress. These results demonstrate that shear stress applied to the surfaces of endothelial cells on collagen gel promotes the growth of microvessel network formation in the gel and expands the network because of repeated bifurcation and elongation.

AB - Shear stress stimulus is expected to enhance angiogenesis, the formation of microvessels. We determined the effect of shear stress stimulus on three-dimensional microvessel formation in vitro. Bovine pulmonary microvascular endothelial cells were seeded onto collagen gels with basic fibroblast growth factor to make a microvessel formation model. We observed this model in detail using phase-contrast microscopy, confocal laser scanning microscopy, and electron microscopy. The results show that cells invaded the collagen gel and reconstructed the tubular structures, containing a clearly defined lumen consisting of multiple cells. The model was placed in a parallel-plate flow chamber. A laminar shear stress of 0.3 Pa was applied to the surfaces of the cells for 48 h. Promotion of microvessel network formation was detectable after ∼10 h in the flow chamber. After 48 h, the length of networks exposed to shear stress was 6.17 (±0.59) times longer than at the initial state, whereas the length of networks not exposed to shear stress was only 3.30 (±0.41) times longer. The number of bifurcations and endpoints increased for networks exposed to shear stress, whereas the number of bifurcations alone increased for networks not exposed to shear stress. These results demonstrate that shear stress applied to the surfaces of endothelial cells on collagen gel promotes the growth of microvessel network formation in the gel and expands the network because of repeated bifurcation and elongation.

KW - Angiogenesis

KW - Blood flow

KW - Capillary

KW - In vitro model

KW - Migration

UR - http://www.scopus.com/inward/record.url?scp=4143135285&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4143135285&partnerID=8YFLogxK

U2 - 10.1152/ajpheart.00400.2003

DO - 10.1152/ajpheart.00400.2003

M3 - Article

C2 - 15130887

AN - SCOPUS:4143135285

VL - 287

JO - American Journal of Physiology - Heart and Circulatory Physiology

JF - American Journal of Physiology - Heart and Circulatory Physiology

SN - 0363-6135

IS - 3 56-3

ER -