The addition of 2-deoxy-D-glucose and quinidine to culture media of strain Fisc of Coprinus macrorhizus was effective in inhibiting fruiting body formation and in reducing the intracellular level of cyclic AMP, adenylate cyclase and phosphodiesterase activities. 2-Deoxy-D-glucose taken into mycelial cells was phosphorylated and accumulated as 2-deoxy-D-glucose-monophosphate and 2-deoxy-D-glucose diphosphate. This phosphorylation appeared to be reversible. Quinidine was taken up by mycelial cells without further metabolism. 2-Deoxy-D-glucose, 2-deoxy-D-glucosemono-phosphate and quinidine inhibited adenylate cyclase activity, but not phosphodiesterase activity measured in vitro. The Ki values of 2-deoxy-D-glucose and quinidine for adenylate cyclase activity were 1.8 and 0.08 mM, respectively. The data indicate a possible relationship between the low adenylate cyclase activity and inhibition of fruiting body formation.
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