Effects of caloric restriction on gene expression in the arcuate nucleus

Isao Shimokawa, Tamaki Fukuyama, Kurumi Yanagihara-Outa, Masato Tomita, Toshimitsu Komatsu, Yoshikazu Higami, Tomoshi Tuchiya, Takuya Chiba, Yoshiharu Yamaza

研究成果: Article

22 引用 (Scopus)


Neuroendocrine alterations that repress energy-costly physiologic processes such as reproduction and growth and induce stress responses, might underlie the antiaging effect of caloric restriction (CR). Neurons in the arcuate nucleus of the hypothalamus (ARH) might have a pivotal role in these neuroendocrine alterations. We investigated the effects of CR on gene expression of neuropeptide Y (NPY), proopiomelanocortin (POMC), growth hormone-releasing hormone (GHRH), somatostatin (SRIH), and cyclophilin (CP) in the ARH in male F344 rats at 6 months of age. Rats were fed ad libitum or a 30% caloric restricted diet with a modified alternate-days feeding regimen from 6 weeks of age. Reverse transcription-polymerase chain reaction (RT-PCR) methods were used to quantify mRNA levels over multiple time points during the 12-h/12-h dark/light cycle over a 2-days feeding cycle. The present study demonstrated that CR increased NPY-mRNA levels, but decreased POMC, GHRH, and CP mRNA levels differentially over the feeding cycle. The SRIH level was not significantly affected by CR. The present results support the neuroendocrine hypothesis of CR.

ジャーナルNeurobiology of Aging
出版物ステータスPublished - 2003 1 1

ASJC Scopus subject areas

  • Neuroscience(all)
  • Ageing
  • Clinical Neurology
  • Developmental Biology
  • Geriatrics and Gerontology

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    Shimokawa, I., Fukuyama, T., Yanagihara-Outa, K., Tomita, M., Komatsu, T., Higami, Y., Tuchiya, T., Chiba, T., & Yamaza, Y. (2003). Effects of caloric restriction on gene expression in the arcuate nucleus. Neurobiology of Aging, 24(1), 117-123. https://doi.org/10.1016/S0197-4580(02)00046-5