Effects of carbocisteine on altered activities of glycosidase and glycosyltransferase and expression of Muc5ac in SO2-exposed rats

Yuji Ishibashi, Fumiyoshi Kobayashi, Akira Idesawa, Akiyoshi Taniguchi, Shigeki Matsuzawa

研究成果: Article査読

21 被引用数 (Scopus)

抄録

Carbocisteine is a mucoregulatory drug regulating fucose and sialic acid contents in mucus glycoprotein. To investigate the mechanism of carbocisteine action, we evaluated the effects of carbocisteine on the activity of fucosidase, sialidase, fucosyltransferase and sialyltransferase, and on the expression of Muc5ac mRNA in the airway epithelium of SO2-exposed rats. Wistar rats were repeatedly exposed to a 300-ppm SO2 gas for 44 days. Carbocisteine (125 and 250 mg/kg ×2/day) was administered for 25 days after 20 days of SO2 gas exposure. These enzyme activities were measured by fluorogenic substrate or glycoproteinic exogenous acceptor method. The expression levels of Muc5ac mRNA and protein were determined with real-time reverse transcriptase-polymerase chain reaction (real-time RT-PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. Carbocisteine (250 mg/kg ×2/day) inhibited all the changes in these enzyme activities and the expressions of Muc5ac mRNA and protein in the lung after repeated SO 2 exposure. These findings suggest that carbocisteine may normalize fucose and sialic acid contents in mucin glycoprotein through regulation of these enzyme activities, and inhibition of both Muc5ac mRNA and protein expressions in SO2-exposed rats.

本文言語English
ページ(範囲)7-15
ページ数9
ジャーナルEuropean Journal of Pharmacology
487
1-3
DOI
出版ステータスPublished - 2004 3 8
外部発表はい

ASJC Scopus subject areas

  • Pharmacology

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