TY - GEN
T1 - Effects of dendrimer-functionalized multi-walled carbon nanotubes on murine embryonic stem cells
AU - Cui, D.
AU - Zhang, H.
AU - Wang, Z.
AU - Toru, A.
AU - Tetsuya, O.
PY - 2008
Y1 - 2008
N2 - Herein we firstly reported the effects of generation 5 polyamidoamine dendrimerfunctionalized fluorescent multi-walled carbon nanotubes(dMNTs) on mice embryonic stem cell line CCE. The dMNTs were prepared and characterized, and incubated with murine ES CCE cells for 1 to 5day. These ES cells were observed by fluorescent microscopy, and were analyzed by flow cytometer and MTT. Results showed that the dendrimer-functionalized fluorescent multi-walled carbon nanotubes were successfully synthesized, could enter into ES cells quickly, more than 20μg/ml dose caused ES cells become smaller and smaller as the incubation time increased, and inhibited cell growth in dose- and time-dependent means, less than 5 μg/ml dose improves ES differentiation. These results demonstrate that dMNTs are toxic to ES cells at large dose, can induce ES cells' differentiation at small dose. The prepared dMNTs may be a highly efficient gene delivery system for ES cells, have potential applications in ES research.
AB - Herein we firstly reported the effects of generation 5 polyamidoamine dendrimerfunctionalized fluorescent multi-walled carbon nanotubes(dMNTs) on mice embryonic stem cell line CCE. The dMNTs were prepared and characterized, and incubated with murine ES CCE cells for 1 to 5day. These ES cells were observed by fluorescent microscopy, and were analyzed by flow cytometer and MTT. Results showed that the dendrimer-functionalized fluorescent multi-walled carbon nanotubes were successfully synthesized, could enter into ES cells quickly, more than 20μg/ml dose caused ES cells become smaller and smaller as the incubation time increased, and inhibited cell growth in dose- and time-dependent means, less than 5 μg/ml dose improves ES differentiation. These results demonstrate that dMNTs are toxic to ES cells at large dose, can induce ES cells' differentiation at small dose. The prepared dMNTs may be a highly efficient gene delivery system for ES cells, have potential applications in ES research.
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U2 - 10.1149/1.2998536
DO - 10.1149/1.2998536
M3 - Conference contribution
AN - SCOPUS:65549159833
T3 - ECS Transactions
SP - 111
EP - 116
BT - ECS Transactions - Fullerenes, Nanotubes, and Carbon Nanostructures - 213th ECS Meeting
PB - Electrochemical Society Inc.
T2 - Fullerenes, Nanotubes, and Carbon Nanostructures - 213th ECS Meeting
Y2 - 18 May 2008 through 23 May 2008
ER -