Effects of dexamethasone on the expression of β1-, β2- and β3-adrenoceptor mRNAs in skeletal and left ventricle muscles in rats

Fuuun Kawano, Jun Tanihata, Shogo Sato, Sachiko Nomura, Akira Shiraishi, Kaoru Tachiyashiki, Kazuhiko Imaizumi

    研究成果: Article

    17 引用 (Scopus)

    抄録

    Glucocorticoids are known to increase the density and mRNA levels of β-adrenoceptors (β-AR) via the glucocorticoid receptor (GR) in many tissues. However, the effects of these changes in the skeletal and cardiac muscles remain relatively unknown. We have investigated the effects of dexamethasone on the expression of the β1-, β2-, and β3-AR mRNAs and GR mRNA in fast-twitch fiber-rich extensor digitorum longus (EDL), slow-twitch fiber-rich soleus (SOL), and left ventricle (LV) muscles by real-time quantitative RT-PCR. Male rats were divided into a dexamethasone group and control group. The weight, RNA concentration, and total RNA content of EDL muscle were 0.76-, 0.85-, and 0.65-fold lower, respectively, in the dexamethasone group than in the control group. The weight, RNA concentration, and total RNA content of SOL muscle were 0.92-, 0.87-, and 0.81-fold lower, respectively, in the dexamethasone group than in the control group; these differences were significant. However, the weight/body weight and total RNA content/body weight of LV muscle were 1.38- and 1.39-fold higher, respectively, in the dexamethasone group than in the control group, respectively; these differences were also significant. Dexamethasone significantly decreased GR mRNA expression in EDL muscle without changing the expression of the β1-, β2-, and β3-AR mRNAs. However, dexamethasone significantly decreased the expressions of β2-AR and GR mRNAs in SOL muscle and significantly increased β1-AR mRNA expression in LV muscle-without changing GR mRNA expression. These results suggest that the effects of dexamethasone on the expression of β1- and β2-AR mRNAs and muscle mass depend on the muscle contractile and/or constructive types.

    元の言語English
    ページ(範囲)383-390
    ページ数8
    ジャーナルJournal of Physiological Sciences
    59
    発行部数5
    DOI
    出版物ステータスPublished - 2009 9

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    Skeletal Muscle Ventricle
    Adrenergic Receptors
    Dexamethasone
    Heart Ventricles
    Messenger RNA
    Glucocorticoid Receptors
    Muscles
    RNA
    Control Groups
    Skeletal Muscle
    Weights and Measures
    Body Weight
    Glucocorticoids
    Real-Time Polymerase Chain Reaction
    Myocardium

    ASJC Scopus subject areas

    • Physiology

    これを引用

    Effects of dexamethasone on the expression of β1-, β2- and β3-adrenoceptor mRNAs in skeletal and left ventricle muscles in rats. / Kawano, Fuuun; Tanihata, Jun; Sato, Shogo; Nomura, Sachiko; Shiraishi, Akira; Tachiyashiki, Kaoru; Imaizumi, Kazuhiko.

    :: Journal of Physiological Sciences, 巻 59, 番号 5, 09.2009, p. 383-390.

    研究成果: Article

    Kawano, Fuuun ; Tanihata, Jun ; Sato, Shogo ; Nomura, Sachiko ; Shiraishi, Akira ; Tachiyashiki, Kaoru ; Imaizumi, Kazuhiko. / Effects of dexamethasone on the expression of β1-, β2- and β3-adrenoceptor mRNAs in skeletal and left ventricle muscles in rats. :: Journal of Physiological Sciences. 2009 ; 巻 59, 番号 5. pp. 383-390.
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    abstract = "Glucocorticoids are known to increase the density and mRNA levels of β-adrenoceptors (β-AR) via the glucocorticoid receptor (GR) in many tissues. However, the effects of these changes in the skeletal and cardiac muscles remain relatively unknown. We have investigated the effects of dexamethasone on the expression of the β1-, β2-, and β3-AR mRNAs and GR mRNA in fast-twitch fiber-rich extensor digitorum longus (EDL), slow-twitch fiber-rich soleus (SOL), and left ventricle (LV) muscles by real-time quantitative RT-PCR. Male rats were divided into a dexamethasone group and control group. The weight, RNA concentration, and total RNA content of EDL muscle were 0.76-, 0.85-, and 0.65-fold lower, respectively, in the dexamethasone group than in the control group. The weight, RNA concentration, and total RNA content of SOL muscle were 0.92-, 0.87-, and 0.81-fold lower, respectively, in the dexamethasone group than in the control group; these differences were significant. However, the weight/body weight and total RNA content/body weight of LV muscle were 1.38- and 1.39-fold higher, respectively, in the dexamethasone group than in the control group, respectively; these differences were also significant. Dexamethasone significantly decreased GR mRNA expression in EDL muscle without changing the expression of the β1-, β2-, and β3-AR mRNAs. However, dexamethasone significantly decreased the expressions of β2-AR and GR mRNAs in SOL muscle and significantly increased β1-AR mRNA expression in LV muscle-without changing GR mRNA expression. These results suggest that the effects of dexamethasone on the expression of β1- and β2-AR mRNAs and muscle mass depend on the muscle contractile and/or constructive types.",
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    AU - Kawano, Fuuun

    AU - Tanihata, Jun

    AU - Sato, Shogo

    AU - Nomura, Sachiko

    AU - Shiraishi, Akira

    AU - Tachiyashiki, Kaoru

    AU - Imaizumi, Kazuhiko

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    KW - Synthesized glucocorticoid dexamethasone

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