Effects of downstream genes on synthetic genetic circuits

Takefumi Moriya, Masayuki Yamamura, Daisuke Kiga

研究成果: Article

4 引用 (Scopus)

抜粋

Background: In order to understand and regulate complex genetic networks in living cells, it is important to build simple and well-defined genetic circuits. We designed such circuits using a synthetic biology approach that included mathematical modeling and simulation, with a focus on the effects by which downstream reporter genes are involved in the regulation of synthetic genetic circuits. Results: Our results indicated that downstream genes exert two main effects on genes involved in the regulation of synthetic genetic circuits: (1) competition for regulatory proteins and (2) protein degradation in the cell. Conclusions: Our findings regarding the effects of downstream genes on regulatory genes and the role of impedance in driving large-scale and complex genetic circuits may facilitate the design of more accurate genetic circuits. This design will have wide applications in future studies of systems and synthetic biology.

元の言語English
記事番号S4
ジャーナルBMC Systems Biology
8
発行部数4
DOI
出版物ステータスPublished - 2014 12 8

ASJC Scopus subject areas

  • Structural Biology
  • Modelling and Simulation
  • Molecular Biology
  • Computer Science Applications
  • Applied Mathematics

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