Effects of downstream genes on synthetic genetic circuits

Takefumi Moriya, Masayuki Yamamura, Daisuke Kiga

研究成果: Article査読

6 被引用数 (Scopus)

抄録

Background: In order to understand and regulate complex genetic networks in living cells, it is important to build simple and well-defined genetic circuits. We designed such circuits using a synthetic biology approach that included mathematical modeling and simulation, with a focus on the effects by which downstream reporter genes are involved in the regulation of synthetic genetic circuits. Results: Our results indicated that downstream genes exert two main effects on genes involved in the regulation of synthetic genetic circuits: (1) competition for regulatory proteins and (2) protein degradation in the cell. Conclusions: Our findings regarding the effects of downstream genes on regulatory genes and the role of impedance in driving large-scale and complex genetic circuits may facilitate the design of more accurate genetic circuits. This design will have wide applications in future studies of systems and synthetic biology.

本文言語English
論文番号S4
ジャーナルBMC Systems Biology
8
4
DOI
出版ステータスPublished - 2014 12 8
外部発表はい

ASJC Scopus subject areas

  • 構造生物学
  • モデリングとシミュレーション
  • 分子生物学
  • コンピュータ サイエンスの応用
  • 応用数学

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