Effects of nitric oxide synthase inhibitors on N-methyl-d-aspartate-induced phase delay of circadian rhythm of neuronal activity in the rat suprachiasmatic nucleus in vitro

Akihito Watanabe, Toshiyuki Hamada, Shigenobu Shibata*, Shigenori Watanabe

*この研究の対応する著者

研究成果: Article査読

66 被引用数 (Scopus)

抄録

Excitatory amino acid (EAA) receptors such as N-methyl-d-aspartate (NMDA) and non-NMDA receptors have been suggested to play an important role in the regulation of photic information from the retina to the suprachiasmatic nucleus (SCN). Therefore, we investigated the role of glutamate as a retinohypothalamic transmitter by analyzing the phase-resetting effects of NMDA and a non-NMDA agonist, (R,S)-α-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA), on the circadian rhythm of SCN firing activity. Nitric oxide (NO) production is believed to be an essential intermediate in NMDA-induced cGMP production in the CNS. Thus, we examined the effects of blockers of NO production on NMDA- or AMPA-induced phase delay of SCN activity rhythm. N-nitro-l-arginine methylester (l-NAME) blocked NMDA- but not AMPA-induced phase shift, indicating the involvement of NO synthesis in NMDA-induced phase changes. l-arginine but not d-arginine caused a phase delay, and l-NAME blocked l-arginine-induced phase delay. In addition, cotreatment with NMDA and l-arginine did not have an additive effect. These results suggest that NO production itself is involved in the phase change of SCN neuron activity, and NMDA-induced phase changes are also mediated via activation of NO synthesis in this nucleus.

本文言語English
ページ(範囲)161-164
ページ数4
ジャーナルBrain Research
646
1
DOI
出版ステータスPublished - 1994 5月 16
外部発表はい

ASJC Scopus subject areas

  • 神経科学(全般)
  • 分子生物学
  • 臨床神経学
  • 発生生物学

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