Effects of polyamines on a continuous cell-free protein synthesis system of an extreme thermophile, Thermus thermophilus

Taketoshi Uzawa, Akihiko Yamagishi, Takuya Ueda, Nobutoshi Chikazumi, Kimitsuna Watanabe, Tairo Oshlma

研究成果: Article査読

16 被引用数 (Scopus)

抄録

A continuous cell-free protein synthesis system of an extremely thermophilic eubacterium, Thermus thermophilus HB27, was constructed. This system produced MS2 phage RNA translation products at a rate of more than 5 μg per hour per 1.9 mg of ribosomes at 65°C and the production continued linearly for at least 340 min. When no polyamine was added, the system did not produce the proteins. The highest activity was recorded when 0.1 mM tetrakis(3-aminopropyl)ammonium and 1.0 mM spermine were added simultaneously.

本文言語English
ページ(範囲)732-734
ページ数3
ジャーナルJournal of biochemistry
114
5
DOI
出版ステータスPublished - 1993 11
外部発表はい

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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