Efficient and stable transformation of Lactuca sativa L. cv. Cisco (lettuce) plastids

Hirosuke Kanamoto, Atsushi Yamashita, Hiroshi Asao, Satoru Okumura, Hisabumi Takase, Masahira Hattori, Akiho Yokota, Ken Ichi Tomizawa

研究成果: Article

109 引用 (Scopus)

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Transgenic plastids offer unique advantages in plant biotechnology, including high-level foreign protein expression. However, broad application of plastid genome engineering in biotechnology has been largely hampered by the lack of plastid transformation systems for major crops. Here we describe the development of a plastid transformation system for lettuce, Lactuca sativa L. cv. Cisco. The transforming DNA carries a spectinomycin-resistance gene (aadA) under the control of lettuce chloroplast regulatory expression elements, flanked by two adjacent lettuce plastid genome sequences allowing its targeted insertion between the rbcL and accD genes. On average, we obtained 1 transplastomic lettuce plant per bombardment. We show that lettuce leaf chloroplasts can express transgene-encoded GFP to ∼36% of the total soluble protein. All transplastomic T0 plants were fertile and the T1 progeny uniformly showed stability of the transgene in the chloroplast genome. This system will open up new possibilities for the efficient production of edible vaccines, pharmaceuticals, and antibodies in plants.

元の言語English
ページ(範囲)205-217
ページ数13
ジャーナルTransgenic Research
15
発行部数2
DOI
出版物ステータスPublished - 2006 4
外部発表Yes

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Biotechnology
  • Food Science

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  • これを引用

    Kanamoto, H., Yamashita, A., Asao, H., Okumura, S., Takase, H., Hattori, M., Yokota, A., & Tomizawa, K. I. (2006). Efficient and stable transformation of Lactuca sativa L. cv. Cisco (lettuce) plastids. Transgenic Research, 15(2), 205-217. https://doi.org/10.1007/s11248-005-3997-2