Efficient depletion of fission yeast condensin by combined transcriptional repression and auxin-induced degradation

Yasutaka Kakui, Frank Uhlmann

研究成果: Chapter

抄録

Structural maintenance of chromosomes (SMC) complexes play pivotal roles in controlling chromatin organization. Condensin is an essential SMC complex that compacts chromatin to form condensed chromosomes in mitosis. Complete condensin inactivation is necessary to reveal how condensin converts interphase chromatin into mitotic chromosomes. Here, we have developed a condensin depletion system in fission yeast that combines transcriptional repression with auxin-inducible protein degradation. This achieves efficient condensin depletion without need for a temperature shift. Our system is useful when studying how condensin contributes to chromosome architecture and is applicable to the study of other SMC complexes.

本文言語English
ホスト出版物のタイトルMethods in Molecular Biology
出版社Humana Press Inc.
ページ25-33
ページ数9
DOI
出版ステータスPublished - 2019
外部発表はい

出版物シリーズ

名前Methods in Molecular Biology
2004
ISSN(印刷版)1064-3745
ISSN(電子版)1940-6029

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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