Efficient gene transfer of high copy number mibilizable plasmids containing a marine Rhodobacter origin of replication

J. Grant Burgess, Hiroaki Sudo, Koji Sode, Tadashi Matsunaga*

*この研究の対応する著者

研究成果: Article査読

2 被引用数 (Scopus)

抄録

The replication region of pRD31, a 3.1-kb endogenous plasmid from a marine Rhodobacter species, has recently been localized and sequenced. We report here incorporation of this replicon into a narrow-host-range mobilizable pBR325 derivative. This has allowed us to establish an efficient conjugative gene transfer system for a hydrogen-producing marine species of Rhodobacter that is unable to grow aerobically. Efficient transfer was obtained (1.2×10-3 transconjugants per recipient cell), and hybrid plasmids replicated with a high copy number (>10) and good stability. Southern hybridization analysis indicated that the new vectors, pRDP203 and pRDP203s, were maintained in marine Rhodobacter sp. NKPB0021 as autonomous replicons without detrimental structural rearrangements, confirming their suitability for use as shuttle vectors.

本文言語English
ページ(範囲)105-108
ページ数4
ジャーナルCurrent Microbiology
26
2
DOI
出版ステータスPublished - 1993 2月
外部発表はい

ASJC Scopus subject areas

  • 微生物学

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