TY - JOUR
T1 - Elasticity mapping of living fibroblasts by AFM and immunofluorescence observation of the cytoskeleton
AU - Haga, Hisashi
AU - Sasaki, Shigeo
AU - Kawabata, Kazushige
AU - Ito, Etsuro
AU - Ushiki, Tatsuo
AU - Sambongi, Takashi
N1 - Funding Information:
This work is partially supported by the Special Funding for Basic Research, Ministry of Education, Science, Sports, and Culture, Japan, to K.K. We thank Mr. Masatsugu Shigeno and Mr. Kazutoshi Watanabe (Seiko Instrument Inc.) for their technical advice.
PY - 2000/2
Y1 - 2000/2
N2 - Using the force mapping mode of atomic force microscopy (AFM), we measured spatial distribution of elastic moduli of living mouse fibroblasts (NIH3T3) in a physiological condition. The nuclear portion of the cellular surface is about 10 times softer than the surroundings. Stiffer fibers are confirmed in the elastic images. In order to investigate origin of the softer nuclear portion and the stiffer fibers, we fixed the identical cells imaged by the AFM, and carried out immunofluorescence observation for three types of cytoskeletal filaments - actin filaments, microtubules, and intermediate filaments, using confocal laser scanning microscopy (CLSM). A comparison between the AFM and the CLSM images revealed that the elasticity of the cells was concerned not only with the distribution of actin network, but also with intermediate filaments, whereas microtubules had no large effect on the measured elasticity. (C) 2000 Elsevier Science B.V.
AB - Using the force mapping mode of atomic force microscopy (AFM), we measured spatial distribution of elastic moduli of living mouse fibroblasts (NIH3T3) in a physiological condition. The nuclear portion of the cellular surface is about 10 times softer than the surroundings. Stiffer fibers are confirmed in the elastic images. In order to investigate origin of the softer nuclear portion and the stiffer fibers, we fixed the identical cells imaged by the AFM, and carried out immunofluorescence observation for three types of cytoskeletal filaments - actin filaments, microtubules, and intermediate filaments, using confocal laser scanning microscopy (CLSM). A comparison between the AFM and the CLSM images revealed that the elasticity of the cells was concerned not only with the distribution of actin network, but also with intermediate filaments, whereas microtubules had no large effect on the measured elasticity. (C) 2000 Elsevier Science B.V.
KW - Atomic force microscopy
KW - Cytoskeleton
KW - Elastic modulus
KW - Fibroblast
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U2 - 10.1016/S0304-3991(99)00157-6
DO - 10.1016/S0304-3991(99)00157-6
M3 - Conference article
C2 - 10741677
AN - SCOPUS:0033979011
SN - 0304-3991
VL - 82
SP - 253
EP - 258
JO - Ultramicroscopy
JF - Ultramicroscopy
IS - 1-4
T2 - The International Conference on Scanning Probe Microscopy, Cantilever Sensors and Nanostructures (SPM '99)
Y2 - 30 May 1999 through 1 June 1999
ER -