Electrochemical Flow Immunoassay Using Capillary Column and Ferrocene Conjugated Immunoglobulin G

Tae Kyu Lim, Noriyuki Nakamura, Masateru Ikehata, Tadashi Matsunaga

研究成果: Article

5 引用 (Scopus)

抄録

This paper describes a miniaturized flow immunoassay system. Ferrocene (Fc) conjugated with anti-HCG immunoglobulin G (IgG) antibody (Fc-IgG) was prepared and used as a novel analytical reagent. The system consists of the immunoreaction part, the capillary column packed with cation exchange resin, and the flow cell for electrochemical detection of Fc-IgG. The assay yielded a linear relationship between signal and HCG concentration in the range of 0-2000 mIU/mL (L = dm3). This electrochemical flow immunoassay requires minute quantities of serum and generates highly reproducible results.

元の言語English
ページ(範囲)872-874
ページ数3
ジャーナルElectrochemistry
68
発行部数11
出版物ステータスPublished - 2000 11
外部発表Yes

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Antibodies
Assays
Ion exchange
Resins
Immunoglobulin G
Positive ions
Cation Exchange Resins
ferrocene

ASJC Scopus subject areas

  • Electrochemistry

これを引用

Lim, T. K., Nakamura, N., Ikehata, M., & Matsunaga, T. (2000). Electrochemical Flow Immunoassay Using Capillary Column and Ferrocene Conjugated Immunoglobulin G. Electrochemistry, 68(11), 872-874.

Electrochemical Flow Immunoassay Using Capillary Column and Ferrocene Conjugated Immunoglobulin G. / Lim, Tae Kyu; Nakamura, Noriyuki; Ikehata, Masateru; Matsunaga, Tadashi.

:: Electrochemistry, 巻 68, 番号 11, 11.2000, p. 872-874.

研究成果: Article

Lim, TK, Nakamura, N, Ikehata, M & Matsunaga, T 2000, 'Electrochemical Flow Immunoassay Using Capillary Column and Ferrocene Conjugated Immunoglobulin G', Electrochemistry, 巻. 68, 番号 11, pp. 872-874.
Lim, Tae Kyu ; Nakamura, Noriyuki ; Ikehata, Masateru ; Matsunaga, Tadashi. / Electrochemical Flow Immunoassay Using Capillary Column and Ferrocene Conjugated Immunoglobulin G. :: Electrochemistry. 2000 ; 巻 68, 番号 11. pp. 872-874.
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KW - HCG

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