Electrochemical Flow Immunoassay Using Capillary Column and Ferrocene Conjugated Immunoglobulin G

Tae Kyu Lim, Noriyuki Nakamura, Masateru Ikehata, Tadashi Matsunaga

研究成果: Article査読

5 被引用数 (Scopus)

抄録

This paper describes a miniaturized flow immunoassay system. Ferrocene (Fc) conjugated with anti-HCG immunoglobulin G (IgG) antibody (Fc-IgG) was prepared and used as a novel analytical reagent. The system consists of the immunoreaction part, the capillary column packed with cation exchange resin, and the flow cell for electrochemical detection of Fc-IgG. The assay yielded a linear relationship between signal and HCG concentration in the range of 0-2000 mIU/mL (L = dm3). This electrochemical flow immunoassay requires minute quantities of serum and generates highly reproducible results.

本文言語English
ページ(範囲)872-874
ページ数3
ジャーナルElectrochemistry
68
11
出版ステータスPublished - 2000 11
外部発表はい

ASJC Scopus subject areas

  • Electrochemistry

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