A microbioassay of vitamin B1 is possible with a new electrode assembly consisting of a platinum anode and a silver peroxide cathode. The response time of the electrode decreases with increasing amount of the bacterial suspension (L. fermenti) injected. A linear relationship is obtained between the steady-state current and the concentration of vitamin B1 in the culture broth. The microbioassay can be completed within 6 h, with relative errors of ±8%. A possible mechanism of the current generation is discussed.
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