Electrochemical probe for on-chip type flow immunoassay: Immunoglobulin G labeled with ferrocenecarboaldehyde

Mina Okochi, Hiroko Ohta, Tsuyoshi Tanaka, Tadashi Matsunaga*

*この研究の対応する著者

研究成果: Article査読

32 被引用数 (Scopus)

抄録

Labeling of ferrocenecarboaldehyde (Fc-CHO) to immunoglobulin G (IgG) via formation of Schiff-base and its reduction was investigated for construction of an electrochemical probe for miniaturized amperometric flow immunoassay. Approximately eight molecules of Fc-CHO were labeled to IgG and the reversible redox property of ferrocene was observed. Labeling efficiency improved by over three times as compared to the conventional method using ferrocenemonocarboxylic acid (Fc-COOH). Also, binding affinity of IgG labeled with Fc-CHO to its antigen, IgE, was investigated by enzyme-linked immunosorbent assay (ELISA) and surface plasmon resonance assay. IgG labeled with Fc-CHO that retained eight ferrocene moiety showed sufficient binding affinity to its antigen and the current response obtained in the flow electrochemical detection system increased by 14-fold as compared with IgG labeled with Fc-COOH when applying the potential of 390 mV vs. Ag/AgCl. The minimum detectable concentration of IgG la-beled with Fc-CHO was 0.06 μm. IgG labeled with Fc-CHO demonstrate biochemical and electrochemical properties that are useful for electrochemical immunosensors.

本文言語English
ページ(範囲)14-19
ページ数6
ジャーナルBiotechnology and Bioengineering
90
1
DOI
出版ステータスPublished - 2005 4月 5
外部発表はい

ASJC Scopus subject areas

  • バイオテクノロジー
  • 微生物学

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