TY - JOUR
T1 - Electrostatics of carboxylated anionic vesicles for improving entrapment capacity
AU - Sou, Keitaro
PY - 2011/3
Y1 - 2011/3
N2 - Electrostatic interaction is an important secondary force affecting the structure, stability, and function of lipid vesicles (liposomes). For this study, a negatively charged lipid with carboxylic acid was mixed with phospholipid to produce anionic vesicles. The electrostatics of the carboxylated anionic vesicle (ca. 200 nm diameter) was determined and correlated with entrapment capacity of the vesicles. Correlative analysis revealed the zeta potential of the vesicles as a factor quantitatively affecting the entrapment capacity for a water-soluble marker, in which the entrapment capacity reached its maximum level in less than -30 mV of zeta potential. Transmission electron microscopy (TEM) revealed that the vesicles with high entrapment capacity are composed of a unilamellar membrane. This finding is expected to be useful for efficient encapsulation of water-soluble pharmaceuticals within vesicles.
AB - Electrostatic interaction is an important secondary force affecting the structure, stability, and function of lipid vesicles (liposomes). For this study, a negatively charged lipid with carboxylic acid was mixed with phospholipid to produce anionic vesicles. The electrostatics of the carboxylated anionic vesicle (ca. 200 nm diameter) was determined and correlated with entrapment capacity of the vesicles. Correlative analysis revealed the zeta potential of the vesicles as a factor quantitatively affecting the entrapment capacity for a water-soluble marker, in which the entrapment capacity reached its maximum level in less than -30 mV of zeta potential. Transmission electron microscopy (TEM) revealed that the vesicles with high entrapment capacity are composed of a unilamellar membrane. This finding is expected to be useful for efficient encapsulation of water-soluble pharmaceuticals within vesicles.
KW - Anionic lipid
KW - Entrapment capacity
KW - Liposome
KW - Phospholipid vesicle
KW - Unilamellar membrane
KW - Zeta potential
UR - http://www.scopus.com/inward/record.url?scp=79952695045&partnerID=8YFLogxK
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U2 - 10.1016/j.chemphyslip.2011.01.002
DO - 10.1016/j.chemphyslip.2011.01.002
M3 - Article
C2 - 21262210
AN - SCOPUS:79952695045
VL - 164
SP - 211
EP - 215
JO - Chemistry and Physics of Lipids
JF - Chemistry and Physics of Lipids
SN - 0009-3084
IS - 3
ER -