TY - JOUR
T1 - ELF electrostimulation on macrophages
T2 - 22nd Annual International Conference of the IEEE Engineering in Medicine and Biology Society
AU - Shimooka, T.
AU - Ushida, K.
AU - Nakata, I.
AU - Ohsaki, K.
AU - Shimizu, K.
PY - 2000/12/1
Y1 - 2000/12/1
N2 - Among the biological effects of ELF electric field, we have been interested in the effect on immune cells. If electric field can modulate the immunoresponse, it will provide a potentially useful technique in clinical practice. The effects of ELF electrostimulation on macrophages were investigated to pursue the possibility of immunomodulation using the apparatus designed for an electric field therapy. First, the current induced in the human body was estimated in a computer simulation and in an actual measurement. The maximum current density in the human body was estimated to be in the order of tens mA/m2 when 10 kV was applied beneath the feet of the subject. The effect of electrostimulation on phagocytosis of macrophages was studied in vitro. The current density of the stimulation was 0.1 or 1 A/m2, and the stimulation duration was 10 sec, 1 min or 1 h. Significant reductions of phagocytosis were observed in stimulated macrophages. There was not significant statistical difference between the cases of 0.1 A/m2 and A/m2, nor among the cases with the different durations of the stimulation. Although it is too early to judge whether the apparatus for electric field therapy is applicable to the immunomodulation, the results of this study showed the possibility that ELF electrostimulation can suppress the activity of macrophages.
AB - Among the biological effects of ELF electric field, we have been interested in the effect on immune cells. If electric field can modulate the immunoresponse, it will provide a potentially useful technique in clinical practice. The effects of ELF electrostimulation on macrophages were investigated to pursue the possibility of immunomodulation using the apparatus designed for an electric field therapy. First, the current induced in the human body was estimated in a computer simulation and in an actual measurement. The maximum current density in the human body was estimated to be in the order of tens mA/m2 when 10 kV was applied beneath the feet of the subject. The effect of electrostimulation on phagocytosis of macrophages was studied in vitro. The current density of the stimulation was 0.1 or 1 A/m2, and the stimulation duration was 10 sec, 1 min or 1 h. Significant reductions of phagocytosis were observed in stimulated macrophages. There was not significant statistical difference between the cases of 0.1 A/m2 and A/m2, nor among the cases with the different durations of the stimulation. Although it is too early to judge whether the apparatus for electric field therapy is applicable to the immunomodulation, the results of this study showed the possibility that ELF electrostimulation can suppress the activity of macrophages.
KW - ELF
KW - Electric field therapy
KW - Phagocytosis of macrophage
KW - Simulation of induced current
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M3 - Conference article
AN - SCOPUS:0034442602
SN - 1557-170X
VL - 2
SP - 1408
EP - 1411
JO - Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference
JF - Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference
Y2 - 23 July 2000 through 28 July 2000
ER -